Different hydrogels were synthesised using lignin and xylan (extracted from rice straw) as the raw material, PVA as matrix template, citric acid, and succinic acid as crosslinkers and were structurally characterised by FT-IR and SEM analysis. Citric acid cross-linked hydrogels caused loose bonding between polymers and resulted in formation of large pores whereas succinic acid cross-linked hydrogels were observed to have less porosity and tight bonding network. The swelling ratio and reswelling capacity of hydrogels revealed that lignin hydrogels outperformed all other hydrogels. All the synthesised hydrogels were tested for their use as soil culture media ingredient for the germination of rice seedlings. All the hydrogels performed well in stress conditions as compared to normal conditions. Xylan/lignin hydrogel and lignin hydrogels performed best under stress conditions. These hydrogels could be used effectively as an ingredient of soil culture media owing to their tendency to absorb and retain water properly.
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http://dx.doi.org/10.1080/14786419.2024.2324463 | DOI Listing |
BMC Mol Cell Biol
January 2025
Institute of Future Biophysics, Institutskiy per. 9, Dolgoprudny, Moscow Oblast, Moscow, Russia.
This paper describes a method for determining the cytotoxicity of chemical compounds based on the detection of fluorescent proteins-in this case, green fluorescent protein (GFP) and red fluorescent protein (RFP), which are released into the medium from dead cells. This method is similar in principle to the lactate dehydrogenase test (LDH test), but it does not require a reaction with a chromogenic substrate. This method also makes it possible to independently determine the viability of different lines when used in cocultures.
View Article and Find Full Text PDFSci Rep
January 2025
Instituto do Coração (InCor), Faculdade de Medicina, Hospital das Clínicas HCFMUSP, Universidade de São Paulo, São Paulo, Brazil.
Doxorubicin-induced cardiomyopathy (DOX-IC) is a significant and common complication in patients undergoing chemotherapy, leading to cardiac remodeling and reduced heart function. We hypothesized that the intrapericardial injection of hydrogels derived from the cardiac decellularized extracellular matrix (dECM) loaded with adipose tissue-derived stromal cells (ASC) and their secretome dampens or reverses the progression of DOX-IC. DOX-IC was induced in Wistar male rats through ten weekly intra-peritoneal injections of doxorubicin (cumulative dose: 18 mg/kg).
View Article and Find Full Text PDFNat Commun
January 2025
State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Blastoids-blastocyst-like structures created in vitro-emerge as a valuable model for early embryonic development research. Non-human primates stem cell-derived blastoids are an ethically viable alternative to human counterparts, yet the low formation efficiency of monkey blastoid cavities, typically below 30%, has limited their utility. Prior research has predominantly utilized embryonic stem cells.
View Article and Find Full Text PDFNat Commun
January 2025
Department of Biomedical Engineering, State University of New York at Binghamton, Binghamton, NY, 13902, USA.
Creating durable, motion-compliant neural interfaces is crucial for accessing dynamic tissues under in vivo conditions and linking neural activity with behaviors. Utilizing the self-alignment of nano-fillers in a polymeric matrix under repetitive tension, here, we introduce conductive carbon nanotubes with high aspect ratios into semi-crystalline polyvinyl alcohol hydrogels, and create electrically anisotropic percolation pathways through cyclic stretching. The resulting anisotropic hydrogel fibers (diameter of 187 ± 13 µm) exhibit fatigue resistance (up to 20,000 cycles at 20% strain) with a stretchability of 64.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
College of Food Science and Engineering, Shaanxi Engineering Research Centre of Dairy Products Quality, Safety and Health, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:
The probiotic encapsulation system has the potential to enhance the prebiotic effects of probiotics. However, challenges arise from the release behavior of this system in vivo and the large size of hydrogel beads. This study aims to address the issues related to the size of previous hydrogel beads and assess the colon-targeted delivery of probiotic polysaccharides composite hydrogel beads (PPHB).
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