AI Article Synopsis
- Fluorescent dyes, used in advanced microscopy, help researchers study biomacromolecules' locations and dynamics with great detail but suffer from issues like photobleaching and photoconversion.
- This review highlights recent developments aimed at improving the photostability of these dyes, particularly focusing on cyanine and rhodamine types.
- The discussion includes how microenvironments and labeling methods impact dye stability and aims to encourage the creation of stronger, more reliable fluorophores for effective live cell imaging.
Article Abstract
Fluorescent dyes synergize with advanced microscopy for researchers to investigate the location and dynamic processes of biomacromolecules with high spatial and temporal resolution. However, the instability of fluorescent dyes, including photobleaching and photoconversion, represent fundamental limits for super-resolution and time-lapse imaging. In this review, we discuss the latest advances in improving the photostability of fluorescent dyes. We summarize the primary photobleaching processes of cyanine and rhodamine dyes and highlight a range of strategies developed in recent years to strengthen these fluorophores. Additionally, we discuss the influence of protein microenvironments and labeling methods on the photostability of fluorophores. We aim to inspire next-generation robust and bright fluorophores that ultimately enable the routine practice of time-lapse super-resolution imaging of live cells.
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| http://dx.doi.org/10.1016/j.cbpa.2024.102439 | DOI Listing |
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