AI Article Synopsis
- A new multiplex real-time PCR technique was created to detect cytomegalovirus (CMV), Epstein-Barr virus (EBV), and herpes simplex virus (HSV) together, while also checking sample quality.
- Testing on 500 blood donors and 320 transfusion recipients showed the assay had a high sensitivity of 100 copies/μL and was 100% consistent with existing commercial tests.
- Out of 820 blood samples, the assay found 34 positive for CMV, 15 for EBV, and 6 for HSV, suggesting that virus testing should be standard for blood transfusions, especially in high-risk groups.
Article Abstract
A multiplex real-time PCR has been developed to simultaneously detect transfusion-transmissible pathogens cytomegalovirus, Epstein-Barr virus and herpes simplex virus, as well as to provide sample quality testing, for the conserved regions of the cytomegalovirus UL123 gene, the Epstein-Barr virus BKRF1 gene, and the herpes simplex virus 1/2 UL30 gene, tested on 500 blood donors and 320 transfusion recipients. The laboratory sensitivities for all 3 pathogens were 100 copies/μL. Compared to the commercial real-time PCR reference kit, the multiplex real-time PCR assay for the detection of CMV, EBV and HSV presented 100% consistency. In 820 whole blood samples, the multiplex real-time PCR assay identified 34 (4.15%) positive for CMV DNA, 15 (1.83%) positive for EBV DNA, and 6 (0.73%) positive for HSV DNA. For blood transfusions in high-risk groups, whole blood herpes virus test should be included in the spectrum of pathogen testing for blood donors and recipients.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2024.116234 | DOI Listing |
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