Alicyclobacillus acidoterrestris is the major threat to fruit juice for its off-odor producing characteristic. In this study, Pyrococcus furiosus Argonaute (PfAgo), a novel endonuclease with precise DNA cleavage activity, was used for A. acidoterrestrisdetection, termed as PAD. The partially amplified 16 S rRNA gene of A. acidoterrestris can be cleaved by PfAgo activated by a short 5'-phosphorylated single strand DNA, producing a new guide DNA (gDNA). Then, PfAgo was activated by the new gDNA to cut a molecular beacon (MB) with fluorophore-quencher reporter, resulting in the recovery of fluorescence. The fluorescent intensity is positively related with the concentration of A. acidoterrestris. The PAD assay showed excellent specificity and sensitivity as low as 10 CFU/mL, which can be a powerful tool for on-site detection of A. acidoterrestris in fruit juice industry in the future, reducing the economic loss.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fm.2024.104475 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Upcycling industrial peach waste to produce dissolving pulp.
Environ Sci Pollut Res Int
January 2025
Laboratory of Design and Development of Innovative Knitted Textiles and Garments, Department of Industrial Design and Production Engineering, University of West Attica, 12244, Egaleo, Attica, Greece.
This study investigates the production of high-purity cellulose pulp from peach (Prunus persica) fruit wastes generated during the processing of a Greek compote and juice production industry. A three-step chemical process is used, including alkaline treatment with NaOH, organic acid (acetic and formic) treatment, and hydrogen peroxide treatment, with the goal of cellulose extraction and purification. A fractional factorial design optimized reagent levels, revealing the strong influence of NaOH concentration on α-cellulose content and degree of polymerization.
View Article and Find Full Text PDFDetermination of Reactive Dyes in Coloring Foodstuff, Fruit Juice Concentrates, and Meat Products by Reductive Azo-Bond Cleavage and LC-ESI-MS/MS Analysis.
J Agric Food Chem
January 2025
Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstraße 20, 38106 Braunschweig, Germany.
Visually appealing foods are often associated by consumers with subjective quality features, such as freshness, palatability, and shelf life. In the past, there have been repeated violations in which regulations on the use of pigments in food were ignored and/or unauthorized or toxic dyes (e.g.
View Article and Find Full Text PDFSocial epidemiology of early adolescent nutrition.
Pediatr Res
January 2025
Department of Pediatrics, University of California, San Francisco, San Francisco, CA, USA.
Background: This study aimed to investigate associations between sociodemographic factors and dietary intake among a diverse population of early adolescents ages 10-13 years in the United States.
Methods: We examined data from the Adolescent Brain Cognitive Development (ABCD) Study in Year 2 (2018-2020, ages 10-13 years, N = 10,280). Multivariable linear regression models were conducted to estimate the adjusted associations between sociodemographic factors (age, sex, race and ethnicity, household income, parental education) and dietary intake of various food groups, measured by the Block Kids Food Screener.
Evaluation of integrated polysaccharide, biopolymers and clay composite membranes for clarification process of citrus fruit (sweet lime) juice.
Int J Biol Macromol
January 2025
Advanced Membrane Technology Research Centre (AMTEC), Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia.
This study focuses on the development of an efficient membrane-based clarification process to enhance the performance of subsequent ultrafiltration and produce high-quality sweet lime juice. A range of casting solutions were prepared using a blend of pore-forming polymers, including polyvinylpyrrolidone (PVP), polyvinylidene fluoride (PVDF), and cellulose acetate (CA), dissolved in dimethylformamide (DMF) solvent through the phase inversion technique. To further enhance the membrane's performance, four biopolymers poly (lactic acid) (PLA), xanthan gum, chitosan, and gelatin were incorporated, with and without clay, to refine its structure, porosity, and surface properties.
View Article and Find Full Text PDFExploring the potential of fruit in RTS beverage: a comprehensive study on preparation and stability.
J Food Sci Technol
January 2025
Department of Grain Science and Technology, CSIR-Central Food Technological Research Institute, Mysuru, 570020 India.
Unlabelled: The potential of (), an underutilized plant rich in medicinal and nutritional value, for producing ready-to-serve (RTS) beverages was explored. The research investigated commercial processing techniques for extracting fruit juice and the impact of stabilizers such as pectin (low and high), xanthan gum, and sodium alginate on beverage quality. Sodium alginate, chosen for its sedimentation rate and sensory acceptability, was further optimized in RTS formulations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!