AI Article Synopsis
- Disruptions in the body's daily rhythms are linked to issues like depression and fatigue, prompting research into measuring hormones like melatonin and cortisol in saliva.* -
- A new method using liquid chromatography-tandem mass spectrometry was developed to measure salivary levels of melatonin, cortisol, and cortisone, requiring only a small saliva sample.* -
- The method was validated with high precision and accuracy, and typical daily patterns of these hormones were found in the saliva of 20 cancer survivors.*
Article Abstract
Disturbances in the diurnal pattern are associated with several clinical and psychological conditions, including depression and fatigue. Salivary sampling for melatonin, cortisol and cortisone provides a non-invasive method for frequent sampling and obtaining biochemical insight into the diurnal pattern of individuals. Therefore, a new liquid chromatography-tandem mass spectrometry-based method for the measurement of salivary melatonin, cortisol and cortisone was developed and validated. The method required 250 μl saliva, used isotope dilution methodology and was based on a liquid-liquid extraction for sample preparation, reversed-phase chromatography and multiple reaction monitoring on a mass spectrometer for quantitation. The lower limits of quantification obtained were 0.010 nmol/L for melatonin, 0.5 nmol/L for cortisol and 1.00 nmol/L for cortisone and the limits of detection were 0.003 nmol/L, 0.15 nmol/L and 0.1 nmol/L respectively. The method imprecision was ≤14% for all measurands, and the method comparison showed highly comparable results with high correlation coefficients (all ≥0.964). Potential interference of cortisol and cortisone by prednisolone was observed and could be detected by chromatogram review. Typical diurnal patterns for melatonin, cortisol and cortisone were observed in the saliva of 20 cancer survivors who collected saliva throughout the day.
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| http://dx.doi.org/10.1016/j.ab.2024.115496 | DOI Listing |
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