Background: The development of sheath blight (ShB) resistance varieties has been a challenge for scientists for long time in rice. Activation tagging is an efficient gain-of-function mutation approach to create novel phenotypes and to identify their underlying genes. In this study, a mutant population was developed employing activation tagging in the recalcitrant indica rice (Oryza sativa L.) cv. BPT 5204 (Samba Mahsuri) through activation tagging.
Methods And Results: In this study, we have generated more than 1000 activation tagged lines in indica rice, from these mutant population 38 (GFP RFP) stable Ds plants were generated through germinal transposition at T generation based on molecular analysis and seeds selected on hygromycin (50 mg/L) containing medium segregation analyses confirmed that the transgene inherited as mendelian segregation ratio of 3:1 (3 resistant: 1 susceptible). Of them, five stable activation tagged Ds lines (M-Ds-1, M-Ds-2, M-Ds-3, M-Ds-4 and M-Ds-5) were selected based on phenotypic observation through screening for sheath blight (ShB) resistance caused by fungal pathogen Rhizoctonia solani (R. solani),. Among them, M-Ds-3 and M-Ds-5 lines showed significant resistance for ShB over other tagged lines and wild type (WT) plants. Furthermore, analysed for launch pad insertion through TAIL-PCR results and mapped on corresponding rice chromosomes. Flanking sequence and gene expression analysis revealed that the upregulation of glycoside hydrolase-OsGH or similar to Class III chitinase homologue (LOC_Os08g40680) in M-Ds-3 and a hypothetical protein gene (LOC_Os01g55000) in M-Ds-5 are potential candidate genes for sheath blight resistance in rice.
Conclusion: In the present study, we developed Ac-Ds based ShB resistance gain-of-functional mutants through activation tagging in rice. These activation tagged mutant lines can be excellent sources for the development of ShB resistant cultivars in rice.
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Article Synopsis
- Sheath blight (ShB), caused by the fungus Rhizoctonia solani, is a serious problem for crops globally, and current rice varieties lack major resistance genes.
- Researchers identified a rice mutant, sbr1, which shows improved resistance to ShB while retaining normal growth traits, though it has an undesirable stay-green characteristic linked to the disruption of the Stay-Green (OsSGR) gene.
- The study found that manipulating cytokinin levels through the knockout of the OsCKX7 gene boosts ShB resistance without negatively affecting yield or causing the stay-green issue, offering new possibilities for developing resistant rice varieties.
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