AI Article Synopsis
- - Decellularization is a technique to extract decellularized extracellular matrix (dECM) for creating 3D scaffolds that mimic the original tissue's structure and properties, aiding in tissue regeneration.
- - The study introduces a new injection-based method for decellularization that does not require a perfusion setup and compares its efficiency between rat and mouse models, utilizing EDTA and SDS for tissue processing.
- - The results demonstrated a significant reduction in DNA content (around 97%) in both mouse and rat dECM, while also preserving a notable proportion of total protein (39% in mice and 65% in rats), maintaining the original tissue's architectural integrity.
Article Abstract
Decellularization is a process to harvest the decellularized extra cellular matrix (dECM) that helps develop 3D scaffolds which mimic the native tissue composition. The decellularized tissues retain the structural and functional properties of the extracellular matrix (ECM) by an efficient decellularization process that retains tissue-specific biochemical and biophysical cues for tissue regeneration. In this study, we report an injection-based decellularization method, without perfusion setup. This study also compares the efficiency of the proposed protocol in the two animal models viz rat and mice. This method harvests rat and mice liver dECM using ethylenediamine tetra acetic acid (EDTA) and sodium dodecyl sulphate (SDS) within 08 h and 02 h respectively and preserved significant amount of ECM proteins. We reported that the harvested mice decellularized extracellular matrix (mdECM) and rat decellularized extracellular matrix (rdECM) had significant reduction in their DNA content (∼97%) and retained structural architecture resembling their native tissue counterparts. The total protein content retained in mdECM was ∼39% while that retained in rdECM was ∼65%. It was also found that the sGAG (sulphated glycosaminoglycan) content showed a no List of Figures.
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| http://dx.doi.org/10.1080/21691401.2024.2319893 | DOI Listing |
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