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Biophysical Characterization and Cryopreservation of Mammalian Cells Using Ionic Liquids. | LitMetric

AI Article Synopsis

  • Ionic liquids (ILs) are versatile solvents that can be customized for specific tasks, making them appealing substitutes for traditional solvents in biological applications.
  • This study focused on carboxylate anion-based ILs to assess their potential as cryoprotectants, which help prevent cell damage during freezing, using two different mammalian cell lines.
  • Results indicated that while ILs generally showed high cytotoxicity, ethylammonium acetate stood out for its effective cryoprotective properties, suggesting that understanding the interactions of IL pairs is crucial for predicting their overall behavior in biological contexts.

Article Abstract

Ionic liquids (ILs) are a diverse class of solvents which can be selected for task-specific properties, making them attractive alternatives to traditional solvents. To tailor ILs for specific biological applications, it is necessary to understand the structure-property relationships of ILs and their interactions with cells. Here, a selection of carboxylate anion-based ILs were investigated as cryoprotectants, which are compounds added to cells before freezing to mitigate lethal freezing damage. The cytotoxicity, cell permeability, thermal behavior, and cryoprotective efficacy of the ILs were assessed with two model mammalian cell lines. We found that the biophysical interactions, including permeability of the ILs, were influenced by considering the IL pair together, rather than as single species acting independently. All of the ILs tested had high cytotoxicity, but ethylammonium acetate demonstrated good cryoprotective efficacy for both cell types tested. These results demonstrate that despite toxicity, ILs may be suitable for certain biological applications. It also demonstrates that more research is required to understand the contribution of ion pairs to structure-property relationships and that knowing the behavior of a single ionic species will not necessarily predict its behavior as part of an IL.

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Source
http://dx.doi.org/10.1021/acs.jpcb.3c06797DOI Listing

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