AI Article Synopsis
- RNA sequencing (RNA-seq) is accurate but expensive and hard to scale, which limits its usage.
- A new method called BOLT-seq offers a more cost-effective and scalable solution for transcriptome profiling using unpurified bulk 3'-end mRNA in crude cell lysates.
- BOLT-seq simplifies the process, allowing for quick library construction and has been successfully used to categorize small molecule drugs by their mechanisms of action.
Article Abstract
The major drawbacks of RNA sequencing (RNA-seq), a remarkably accurate transcriptome profiling method, is its high cost and poor scalability. Here, we report a highly scalable and cost-effective method for transcriptomics profiling called Bulk transcriptOme profiling of cell Lysate in a single poT (BOLT-seq), which is performed using unpurified bulk 3'-end mRNA in crude cell lysates. During BOLT-seq, RNA/DNA hybrids are directly subjected to tagmentation, and second-strand cDNA synthesis and RNA purification are omitted, allowing libraries to be constructed in 2 h of hands-on time. BOLT-seq was successfully used to cluster small molecule drugs based on their mechanisms of action and intended targets. BOLT-seq competes effectively with alternative library construction and transcriptome profiling methods.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10907608 | PMC |
| http://dx.doi.org/10.1038/s12276-024-01164-8 | DOI Listing |
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