The synthesis of six model trisaccharides representative of galactomannans produced by lichens was performed through stereoselective glycosylation. These standards include linear and branched galactomannans bearing either galactofuranosyl or galactopyranosyl entities. The complete assignment of H and C signals for both forms of synthetically reduced oligosaccharides was performed. The resulting NMR data were used to quickly demonstrate the structural characteristics of minor polysaccharides within different extracts of three representative lichens.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/d4ob00047a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Green preparation of low-molecular-weight galactomannan from Gleditsia sinensis and mechanistic investigation on ameliorating nonalcoholic fatty liver disease.
Food Res Int
February 2025
Department of Chemistry and Chemical Engineering, State Key Laboratory of Efficient Production of Forest Resources, Engineering Research Center of Forestry Biomass Materials and Bioenergy (Ministry of Education), Beijing Forestry University, Beijing 100083, China. Electronic address:
Galactomannan comes from a wide range of plant resources and has some biological activities, but its bioavailability is limited due to its large molecular weight and complex structure. In this study, three degradation methods (HO, ultrasound, and β-mannanase) combined with ethanol fractional precipitation (25 %, 50 %, and 75 %) were used to degrade and separate Gleditsia sinensis galactomannans (GSG), and the physicochemical properties and biological activities of GSG after degradation were analyzed. Comprehensive comparison indicates that HO exhibits had a better degradation effect.
View Article and Find Full Text PDFIsolation of a Novel Low-Temperature-Active and Organic-Solvent-Stable Mannanase from the Intestinal Metagenome of .
Int J Mol Sci
December 2024
Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
The black soldier fly, , is a voracious scavenger of various organic materials; therefore, it could be exploited as a biological system for processing daily food waste. In order to survey novel hydrolytic enzymes, we constructed a fosmid metagenome library using unculturable intestinal microorganisms from . Through functional screening of the library on carboxymethyl cellulose plates, we identified a fosmid clone, the product of which displayed hydrolytic activity.
View Article and Find Full Text PDFCovalent Modification of Selenium in Polysaccharide Enhances Immunoregulation Activity via the TLR4-Mediated MAPK/NF-κB Signaling Pathway.
J Agric Food Chem
January 2025
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China.
Selenium (Se) is a crucial trace element that demonstrates significant immunomodulatory effects, which are attributed to the variability in its valence states and metabolic pathways. To investigate the Se-related immunoregulatory effects, locust bean gum (LBG), a typical galactomannan, was selenized by employing deep eutectic solvents (DESs) as high-efficiency solvents to obtain Se-covalent modified LBG (SeLBGs) with similar molecular mass and different Se contents (SeLBG, 1049.57 and SeLBG, 4926.
View Article and Find Full Text PDFSynthesis of branched heterooligosaccharides related to Aspergillus galactomannan containing short Galf side chains.
Carbohydr Res
March 2025
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospekt, 119991, Moscow, Russian Federation. Electronic address:
The members of a widespread Aspergillus fungi genus cause various diseases including the invasive aspergillosis with high morbidity and mortality rates, especially for immunosuppressed patients. One of the main carbohydrate structures on the surface of their cell wall is the galactomannan (GM) which is used in diagnostic kits for the detection of specific types of aspergillosis. However, limited specificity of currently available test systems urges the need for their further improvement.
View Article and Find Full Text PDFIdentification of novel fructo-oligosaccharide bacterial consumers by pulse metatranscriptomics in a human stool sample.
mSphere
December 2024
Department of Pharmacology & Therapeutics, Faculty of Medicine and Health Sciences, McGill University, Montréal, Québec, Canada.
Unlabelled: Dietary fibers influence the composition of the human gut microbiota and directly contribute to its downstream effects on host health. As more research supports the use of glycans as prebiotics for therapeutic applications, the need to identify the gut bacteria that metabolize glycans of interest increases. Fructo-oligosaccharide (FOS) is a common diet-derived glycan that is fermented by the gut microbiota and has been used as a prebiotic.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!