AI Article Synopsis
- The chapter outlines a method for isolating microsomes from the hypocotyl tissue of dark-grown Arabidopsis thaliana.
- Microsomes are mainly made up of membranes from the endoplasmic reticulum and appear as vesicle-like structures.
- The authors detail the experimental process, including steps for sample preparation, homogenization, and differential centrifugation, along with quality control measures post-isolation.
Article Abstract
In this book chapter, we present a method for microsome isolation from the hypocotyl tissue of dark-grown Arabidopsis thaliana. Microsomes are heterogeneous, vesicle-like membranes, which are, not exclusively, derived but enriched with membranes of the endoplasmic reticulum (ER). Here, we describe the experimental setup, including sample preparation, homogenization, differential centrifugation steps, and quality control measures after microsome isolation.
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| http://dx.doi.org/10.1007/978-1-0716-3710-4_29 | DOI Listing |
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