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and negatively regulate the sugar metabolism of Mill. | LitMetric

and negatively regulate the sugar metabolism of Mill.

Front Plant Sci

National-Local Joint Engineering Laboratory of High Efficiency and Superior Quality Cultivation and Fruit Deep Processing Technology on Characteristic Fruit Trees, Alar, Xinjiang, China.

Published: February 2024

Hexokinase (HXK) plays a crucial role in plants, catalyzing the phosphorylation of hexose substances, which is one of the key steps in sugar metabolism and energy production. While genes have been well-studied in model plants, the evolutionary and functional characteristics of HXK gene family in jujube is unknow. In this study, the gene family members were identified by bioinformatics methods, the key members regulating glucose metabolism were identified by transcriptome data, and finally the function of the key genes was verified by instantaneous and stable genetic transformation. Our results showed that seven genes were identified in the jujube genome, all of which were predict located in the chloroplast and contain Hexokinase-1 (PF00349) and Hexokinase-2 (PF03727) conserved domains. Most of HXK proteins were transmembrane protein with stable, lipid-soluble, hydrophilic. The secondary structure of ZjHXK proteins main α-helix, and contains two distinct tertiary structure. All genes contain nine exons and eight introns. Predictions of cis-regulatory elements indicate that the promoter region of ZjHXK contains a large number of MeJA responsive elements. Finally, combined with the analysis of the relationship between the expression and glucose metabolism, found that and may the key genes regulating sugar metabolism. Transient overexpression of and on jujube, or allogeneic overexpression of and on tomato would significantly reduce the content of total sugar and various sugar components. Transient silencing of and genes results in a significant increase in sucrose and total sugar content. Interestingly, the expression of and were also affected by methyl jasmonate.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10895003PMC
http://dx.doi.org/10.3389/fpls.2024.1335120DOI Listing

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