AI Article Synopsis
- This study focuses on Burkholderia dolosa, a dangerous pathogen that can cause infections in hospitalized patients, especially those with strokes. A specific isolate, B. dolosa N149, was characterized as highly resistant to many antibiotics and exceptionally virulent.
- Using advanced genetic techniques, the researchers identified that this strain had resistance to 31 antibiotics and showcased various mechanisms like multidrug efflux pumps that contribute to its drug resistance.
- The genome analysis revealed numerous virulence genes linked to its ability to cause disease, and B. dolosa N149 was classified as a new sequence type, furthering understanding of its genetic diversity and potential threat in clinical settings.
Article Abstract
Objectives: Burkholderia dolosa is a clinically important opportunistic pathogen in inpatients. Here we characterised an extensively drug-resistant and hypervirulent B. dolosa isolate from a patient hospitalised for stroke.
Methods: Resistance to 41 antibiotics was tested with the agar disc diffusion, minimum inhibitory concentration, or broth microdilution method. The complete genome was assembled using short-reads and long-reads and the hybrid de novo assembly method. Allelic profiles obtained by multilocus sequence typing were analysed using the PubMLST database. Antibiotic-resistance and virulence genes were predicted in silico using public databases and the 'baargin' workflow. B. dolosa N149 phylogenetic relationships with all available B. dolosa strains and Burkholderia cepacia complex strains were analysed using the pangenome obtained with Roary.
Results: B. dolosa N149 displayed extensive resistance to 31 antibiotics and intermediate resistance to 4 antibiotics. The complete genome included three circular chromosomes (6 338 630 bp in total) and one plasmid (167 591 bp). Genotypic analysis revealed various gene clusters (acr, amr, amp, emr, ade, bla and tet) associated with resistance to 35 antibiotic classes. The major intrinsic resistance mechanisms were multidrug efflux pump alterations, inactivation and reduced permeability of targeted antibiotics. Moreover, 91 virulence genes (encoding proteins involved in adherence, formation of capsule, biofilm and colony, motility, phagocytosis inhibition, secretion systems, protease secretion, transmission and quorum sensing) were identified. B. dolosa N149 was assigned to a novel sequence type (ST2237) and formed a mono-phylogenetic clade separated from other B. dolosa strains.
Conclusions: This study provided insights into the antimicrobial resistance and virulence mechanisms of B. dolosa.
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| http://dx.doi.org/10.1016/j.jgar.2024.02.009 | DOI Listing |
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