The combined effect of ultrafine particles of cobalt and manganese oxides and herb extract on ruminal digestion .

Background And Aim: At present, detailed studies are being conducted to confirm the safety of the use of metal-containing ultrafine particles (UFP) in animal feeding, preventing the possibility of negative effects on productive qualities and physiological state, as well as on the environment and final consumer, that is, humans. Thus, the purpose of this research was to study the safety of cobalt- and manganese-containing UFP (UFP CoO, MnO UFP) together with (PB) herb extract in a bioluminescence inhibition test, as well as the effect of this composition on ruminal digestion .

Materials And Methods: The safety of the studied samples was determined using a multifunctional microplate analyzer TECAN Infinite F200 (Tecan Austria GmbH, Austria), recording the luminescence value of the bacterial strain (Ecolum, JSC NVO Immunotech, Russia). Dry matter (DM) digestibility studies were performed using the method on an "artificial rumen" model using an ANKOM Daisy II incubator unit (AD II; USA). The number of protozoa in ruminal fluid was counted in a Goryaev chamber. The bacterial mass was assessed by differential centrifugation followed by drying. This method is based on differences in the sedimentation rate of particles that differ in size and density.

Results: UFP CoO and MnO at concentrations above 1.5 × 10 and 1.9 × 10 mol/L, respectively, have a pronounced bactericidal effect, suppressing more than 50% of the luminescence of . The combined use of UFP metals and plant extract increases the luminescence of the test object, indicating its safety. The combined use of UFP and PB increases the digestibility of feed DM and the number of protozoa in 1 mL of ruminal fluid; however, the combination of UFP MnO + PB (13.8%) yielded the best result, which is recommended for further research.

Conclusion: extract reduces the toxicity of UFP CoO and MnO , indicating that their combined use is safer.