We present a fully automatic montage pipeline for adaptive optics SLO retinal images. It contains a flexible module to estimate the translation between pairwise images. The user can change modules to accommodate the alignment of the dataset using the most appropriate alignment technique, provided that it estimates the translation between image pairs and provides a quantitative confidence metric for the match between 0 and 1. We use these pairwise comparisons and associated metrics to construct a graph where nodes represent frames and edges represent the overlap relations. We use a small diameter spanning tree to determine the best pairwise alignment for each image based on the entire set of image relations. The final stage of the pipeline is a blending module that uses dynamic programming to improve the smoothness of the transition between frames. Data sets ranging from 26 to 119 images were obtained from individuals aged 24 to 81 years with a mix of visually normal control eyes and eyes with glaucoma or diabetes. The resulting automatically generated montages were qualitatively and quantitatively compared to results from semi-automated alignment. Data sets were specifically chosen to include both high quality and medium quality data. The results obtained from the automatic method are comparable or better than results obtained by an experienced operator performing semi-automated montaging. For the plug-in pairwise alignment module, we tested a technique that utilizes SIFT + RANSAC, Normalized cross-correlation (NCC) and a combination of the two. This pipeline produces consistent results not only on outer retinal layers, but also on inner retinal layers such as a nerve fiber layer or images of the vascular complexes, even when images are not of excellent quality.
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http://dx.doi.org/10.1364/BOE.505013 | DOI Listing |
Proc Natl Acad Sci U S A
January 2025
Section on Perception, Cognition, Action, Laboratory of Sensorimotor Research, National Eye Institute, NIH, Bethesda, MD 20892.
To what extent does concept formation require language? Here, we exploit color to address this question and ask whether macaque monkeys have color concepts evident as categories. Macaques have similar cone photoreceptors and central visual circuits to humans, yet they lack language. Whether Old World monkeys such as macaques have consensus color categories is unresolved, but if they do, then language cannot be required.
View Article and Find Full Text PDFPLoS One
January 2025
Faculty of Philosophy, Philosophy of Science and the Study of Religion, Ludwig Maximilian University of Munich, München, Germany.
Many visualisations used in the climate communication field aim to present the scientific models of climate change to the public. However, relatively little research has been conducted on how such data are visually processed, particularly from a behavioural science perspective. This study examines trends in visual attention to climate change predictions in world maps using mobile eye-tracking while participants engage with the visualisations.
View Article and Find Full Text PDFTraffic Inj Prev
January 2025
National Key Laboratory of Human Factors Engineering, China Astronaut Research and Training Centre, Beijing, China.
Objective: Attention forms the foundation for the formation of situation awareness. Low situation awareness can lead to driving performance decline, which can be dangerous in driving. The goal of this study is to investigate how different types of pre-takeover tasks, involving cognitive, visual and physical resources engagement, as well as individual attentional function, affect driver's attention restoration in conditionally automated driving.
View Article and Find Full Text PDFAnal Chem
January 2025
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, Hunan 414006, China.
Diffraction imaging of cells allows rapid phenotyping by the response of intracellular molecules to coherent illumination. However, its ability to distinguish numerous types of human leukocytes remains to be investigated. Here, we show that accurate classification of three lymphocyte subtypes can be achieved with features extracted from cross-polarized diffraction image (p-DI) pairs.
View Article and Find Full Text PDFNanomaterials (Basel)
December 2024
Emerging Technologies Research Center, XPANCEO, Internet City, Emmay Tower, Dubai, United Arab Emirates.
Due to their high refractive index, record optical anisotropy and a set of excitonic transitions in visible range at a room temperature, transition metal dichalcogenides have gained much attention. Here, we adapted a femtosecond laser ablation for the synthesis of WSe nanoparticles (NPs) with diameters from 5 to 150 nm, which conserve the crystalline structure of the original bulk crystal. This method was chosen due to its inherently substrate-additive-free nature and a high output level.
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