, a Gram-negative bacterium widely found in freshwater environments, acts as a common conditional pathogen affecting humans, livestock, and aquatic animals. In this study, the impact of oridonin, an -kaurane diterpenoid compound derived from , on the virulence factors of AS 1.1801 and its antibacterial mechanism was elucidated. The minimum inhibitory concentration (MIC) of oridonin against AS 1.1801 was 100 μg/mL. Oridonin at inhibitory concentrations could significantly increase the electrical conductivity in the supernatant and escalate nucleic acid leakage ( < 0.01). This effect was concomitant with observed distortions in bacterial cells, the formation of cytoplasmic cavities, cellular damage, and pronounced inhibition of protein and nucleic acid synthesis. Additionally, oridonin at inhibitory levels exhibited a noteworthy suppressive impact on AS 1.1801 across biofilm formation, motility, hemolytic activity, lipase activity, and protease activity ( < 0.05), demonstrating a dose-dependent enhancement. qRT-PCR analysis showed that the gene expression of , and were significantly downregulated after oridonin treatment in AS 1.1801 ( < 0.05). Our results indicated that oridonin possessed significant antibacterial and anti-virulence effects on AS 1.1801.
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