The objective of this study was to compare, by qPCR, the circulating blood parasite load of in the buffy coat, and in whole blood mixed with boiled and unboiled guanidine hydrochloride-EDTA buffer, of individuals with chronic ChD. The concentration and purity of DNA were evaluated in a Nanodrop Denovix DS-11FX Series Spectrophotometer (DeNovix Inc., Wilmington, NC, USA). The parasite load was determined with the Taqman qPCR system using a Stratagene Mx3000P thermocycler (Agilent Technologies, Santa Clara, CA, USA) with Cruzi 1 and Cruzi 2 satellite primers. Student's -test with Bonferroni correction, Chi-squared (χ) tests and Spearman's correlation coefficient were applied. The concentration and purity of DNA were higher in the buffy coat. Parasite DNA was detected and quantifiable in the three types of samples in seven patients, without statistically significant differences in the parasite load obtained. Higher correlations were found between the total DNA concentrations and the parasite loads obtained in the samples of the buffy coat.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10893161 | PMC |
| http://dx.doi.org/10.3390/microorganisms12020249 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Functional MRGPRX2 expression on peripheral blood-derived human mast cells increases at low seeding density and is suppressed by interleukin-9 and fetal bovine serum.
Front Immunol
December 2024
KU Leuven Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Leuven, Belgium.
Primary human mast cells (MC) obtained through culturing of blood-derived MC progenitors are the preferred model for the study of MRGPRX2- IgE-mediated MC activation. In order to assess the impact of culture conditions on functional MRGPRX2 expression, we cultured CD34-enriched PBMC from peripheral whole blood (PB) and buffy coat (BC) samples in MethoCult medium containing stem cell factor (SCF) and interleukin (IL)-3, modified through variations in seeding density and adding or withholding IL-6, IL-9 and fetal bovine serum (FBS). Functional expression of MRGPRX2 was assessed after 4 weeks via flow cytometry.
View Article and Find Full Text PDFEpidemiological investigation of trypanosomosis in livestock and distribution of vector in Dabo Hana district, Southwest Oromia, Ethiopia.
Parasite Epidemiol Control
November 2024
Ethiopian Institute of Agricultural Research, Tepi Agricultural Research Center, P.O. Box 34, Tepi, Ethiopia.
The trypanosomosis remains unresolved due to its impact on various hosts, leading to production losses in Ethiopia. In the Southwest of Oromia, multiple livestock species share grazing land in tsetse-infested areas. Thus, a cross-sectional study was conducted from December 2020 to December 2021 to determine the prevalence and associated risk factors of trypanosomosis in bovines, small ruminants, and equines, as well as the distribution of the vector in the Dabo Hana district of Southwest Oromia, Ethiopia.
View Article and Find Full Text PDFPurified Granulocyte Concentrates from Buffy Coats with Extended Storage Time.
Transfus Med Hemother
December 2024
Artcline GmbH, Rostock, Germany.
Article Synopsis
- Granulocyte concentrates (GCs) can be made from pooled buffy coats of whole blood donations, allowing for better availability and longer storage times compared to those made from single-donor apheresis, which can only last 24 hours.
- A process was developed to significantly reduce red blood cell and platelet contamination, extending the shelf life of GCs up to 72 hours while maintaining high cell viability (above 98%) and functionality (with over 95% rates of phagocytosis and oxidative burst).
- To produce a therapeutic dose of GCs, around 15-20 buffy coats are needed, offering a more efficient alternative for treatment compared to traditional methods.
Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors.
Cell Stress
November 2024
National Heart and Lung Institute, Imperial College London London, W12 ONN UK.
Human peripheral blood mononuclear cells (PBMCs) are used to examine biological processes and disease, when basal variability in cellular activation and splicing is described and unexplained. Using isolation systems that maintained buffy coat cells (PBMCs, platelets) in their own plasma, poly-A enriched RNA-sequencing (RNASeq) detected 42,720 Ensembl gene IDs, including >95% of the top 100 Genotype Tissue Expression Project (GTEx)-expressed genes in lung, colon, heart, skeletal muscle and liver, and 10/17 clinically-actionable genes listed by the Pharmacogenomics Knowledgebase. Transcriptome changes were defined after 1h treatment with 32°C hypothermia (hsp70 family member change), 10 μmol/L ferric citrate that had no discernible effect, and 100 μg/mL cycloheximide leading to induction of primary response (immediate early) genes including IL1B and TNF.
View Article and Find Full Text PDFPolygenic Score for Clinicopathologic Features and Survival Outcomes in Papillary Thyroid Carcinoma.
JAMA Otolaryngol Head Neck Surg
November 2024
Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston.
Importance: Genome-wide association studies have identified germline variants associated with the development of papillary thyroid carcinoma (PTC) that can be used to construct a polygenic score (PGS). It is important to determine whether patients with higher germline genetic risk, as summarized using PGS, present with more aggressive disease and/or develop worse clinical outcomes.
Objective: To assess whether germline risk defined by PGS is associated with clinicopathologic features and survival outcomes for patients with PTC.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!