In-Depth Genome Characterization and Pan-Genome Analysis of Strain KMM 296, a Producer of Highly Active Alkaline Phosphatase; Proposal for the Reclassification of and as the Later Heterotypic Synonyms of and , and Emended Description of the Species and .

A strictly aerobic, Gram-stain-negative, rod-shaped, and motile bacterium, designated strain KMM 296, isolated from the coelomic fluid of the mussel , was investigated in detail due to its ability to produce a highly active alkaline phosphatase CmAP of the structural family PhoA. A previous taxonomic study allocated the strain to the species , a member of the family of the class . However, 16S rRNA gene sequencing showed KMM 296's relatedness to NRIC 0815. The isolate grew with 0.5-19% NaCl at 4-42 °C and hydrolyzed Tweens 20 and 40 and L-tyrosine. The DNA G+C content was 62.5 mol%. The prevalent fatty acids were C ω7c, C 3-OH, C ω7c, C, and C cyclo. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidic acid, and also an unidentified aminolipid, phospholipid, and a few unidentified lipids. The major respiratory quinone was Q-8. According to phylogenomic and chemotaxonomic evidence, and the nearest neighbors, the strain KMM 296 represents a member of the species . The genome-based analysis of NRIC 0815 and NRIC 0814 showed their belonging to a single species. In addition, the high similarity between the NRIC 0813 and LMG 2217 genomes suggests their affiliation to one species. Based on the rules of priority, should be reclassified as a later heterotypic synonym of , and is a later heterotypic synonym of . The emended descriptions of the species and are also proposed.