AI Article Synopsis
- * Structural characterization suggests RLPa-2 has specific linkages that contribute to its molecular configuration, potentially impacting its biological functions.
- * When tested in vitro, RLPa-2 showed a significant reduction in M1 macrophage markers and pro-inflammatory mediators, indicating its potential as an anti-inflammatory treatment.
Article Abstract
RLPa-2 (Mw 15.6 kDa) is a polysaccharide isolated from . It consists of arabinose (Ara), galactose (Gal), rhamnose (Rha), glucose (Glc), xylose (Xyl), and galacturonic acid (Gal-UA) with a molar ratio of 1.00:0.91:0.39:0.34:0.25:0.20. Structural characterization was performed by methylation and NMR analysis, which indicated that RLPa-2 might comprise →6)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→, α-L-Araf-(1→, →2,4)-α-D-Glcp-(1→, β-D-Xylp, and α-L-Rhap. In addition, the bioactivity of RLPa-2 was assessed through an in vitro macrophage polarization assay. Compared to positive controls, there was a significant decrease in the expression of M1 macrophage markers (CD80, CD86) and p-STAT3/STAT3 protein. Additionally, there was a down-regulation in the production of pro-inflammatory mediators (NO, IL-6, TNF-α), indicating that M1 macrophage polarization induced with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) stimulation could be inhibited by RLPa-2. These findings demonstrate that the RLPa-2 might be considered as a potential anti-inflammatory drug to reduce inflammation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10888661 | PMC |
| http://dx.doi.org/10.3390/ijms25042133 | DOI Listing |
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