as a Negative Regulator of Avian Adipocyte Differentiation: Implications for Fat Deposition.

The NOTCH signaling pathway plays a pivotal role in diverse developmental processes, including cell proliferation and differentiation. In this study, we investigated whether this signaling molecules also contribute to avian adipogenesis. Using previous mRNA-seq datasets, we examined the expression of 11 signaling members during avian adipocyte differentiation. We found most members are down-regulated throughout differentiation ( < 0.05). As a representative, was decreased in cultured chicken abdominal adipocytes during adipogenesis at mRNA and protein levels ( < 0.05). Moreover, using an overexpression plasmid for NOTCH1's intracellular domain (NICD1), as well as siRNA and DAPT to activate or deplete in cells, we investigated the role of in avian adipogenesis. Our findings illuminate that activates the expression of and while it decreases and ( < 0.05), as well as inhibits oleic acid-induced adipocyte differentiation ( < 0.01). We further demonstrate that , a downstream transcription factor activated by , also significantly inhibits adipogenesis by suppressing and ( < 0.01). Collectively, these findings establish as a negative regulator of avian adipocyte differentiation, unveiling NOTCH signaling as a potential target for regulating avian fat deposition.