Objectives: Recently, reports on antimicrobial-resistant Bacteroides and Prevotella isolates have increased in the Netherlands. This urged the need for a surveillance study on the antimicrobial susceptibility profile of Bacteroides, Phocaeicola, Parabacteroides and Prevotella isolates consecutively isolated from human clinical specimens at eight different Dutch laboratories.
Methods: Each laboratory collected 20-25 Bacteroides (including Phocaeicola and Parabacteroides) and 10-15 Prevotella isolates for 3 months. At the national reference laboratory, the MICs of amoxicillin, amoxicillin/clavulanic acid, piperacillin/tazobactam, meropenem, imipenem, metronidazole, clindamycin, tetracycline and moxifloxacin were determined using agar dilution. Isolates with a high MIC of metronidazole or a carbapenem, or harbouring cfiA, were subjected to WGS.
Results: Bacteroides thetaiotaomicron/faecis isolates had the highest MIC90 values, whereas Bacteroides fragilis had the lowest MIC90 values for amoxicillin/clavulanic acid, piperacillin/tazobactam, meropenem, imipenem and moxifloxacin. The antimicrobial profiles of the different Prevotella species were similar, except for amoxicillin, for which the MIC50 ranged from 0.125 to 16 mg/L for Prevotella bivia and Prevotella buccae, respectively. Three isolates with high metronidazole MICs were sequenced, of which one Bacteroides thetaiotaomicron isolate harboured a plasmid-located nimE gene and a Prevotella melaninogenica isolate harboured a nimA gene chromosomally.Five Bacteroides isolates harboured a cfiA gene and three had an IS element upstream, resulting in high MICs of carbapenems. The other two isolates harboured no IS element upstream of the cfiA gene and had low MICs of carbapenems.
Conclusions: Variations in resistance between species were observed. To combat emerging resistance in anaerobes, monitoring resistance and conducting surveillance are essential.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10984934 | PMC |
| http://dx.doi.org/10.1093/jac/dkae043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Gut microbiota and metabolic responses to a 12-week caloric restriction combined with strength and HIIT training in patients with obesity: a randomized trial.
BMC Sports Sci Med Rehabil
December 2024
Department of Biological and Medical Science, Faculty of Physical Education and Sport, Comenius University in Bratislava, Bratislava, 814 69, Slovakia.
Background: Nowadays, obesity has become a major health issue. In addition to negatively affecting body composition and metabolic health, recent evidence shows unfavorable shifts in gut microbiota in individuals with obesity. However, the effects of weight loss on gut microbes and metabolites remain controversial.
View Article and Find Full Text PDFPhenolics-Rich Extract from Agarwood Leaf-Tea Alleviate Dextran Sulfate Sodium (DSS)-Induced Ulcerative Colitis Via Modulating Intestinal Barrier Function, Liver Inflammation, and Gut Microbiota.
Mol Nutr Food Res
December 2024
School of Food Science and Engineering, Hainan University, Haikou, 570228, P. R. China.
Scope: At present, the incidence rate of ulcerative colitis (UC) continues to increase, causing a global burden. In addition, therapeutic drugs have great side effects. According to modern pharmacology, agarwood leaves have anti-inflammatory, antibacterial, hypoglycemic, and lipid-lowering effects.
View Article and Find Full Text PDFSafety and efficacy assessment of fecal microbiota transplantation as an adjunctive treatment for IgA nephropathy: an exploratory clinical trial.
Sci Rep
October 2024
The Nephrology Department of Shanxi Provincial People's Hospital, Shanxi Medical University, Taiyuan, 030001, China.
Article Synopsis
- A study was done to see if fecal microbiota transplantation (FMT) is safe and helps treat IgA nephropathy (IgAN), a kidney disease.
- Fifteen patients tried FMT using special capsules, and their health indicators were checked before and after the treatment.
- The results showed no serious side effects, and patients had lower levels of protein in their urine afterward, which suggests that FMT might help improve their condition.
Molecular characterization and antibiotic resistance of clinical Bacteroides and related genera from a tertiary care center in Türkiye (Turkey).
Anaerobe
December 2024
Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkiye; ESCMID Study Group for Anaerobic Infections (ESGAI), Basel, Switzerland. Electronic address:
Objectives: This study was conducted to measure the prevalence of antibiotic resistance, and corresponding resistance genes among Bacteroides and related genera in a tertiary hospital.
Methods: We examined 138 clinical strains of Bacteroides, Phocaeicola and Parabacteroides species isolated between July 2018 and June 2022. Antibiotic susceptibility tests were conducted using agar dilution.
Comparative Binding Study of Gliptins to Bacterial DPP4-like Enzymes for the Treatment of Type 2 Diabetes Mellitus (T2DM).
Int J Mol Sci
May 2024
ProtoQSAR SL, CEEI (Centro Europeo de Empresas Innovadoras), Parque Tecnológico de Valencia, 46980 Valencia, Spain.
The role of the gut microbiota and its interplay with host metabolic health, particularly in the context of type 2 diabetes mellitus (T2DM) management, is garnering increasing attention. Dipeptidyl peptidase 4 (DPP4) inhibitors, commonly known as gliptins, constitute a class of drugs extensively used in T2DM treatment. However, their potential interactions with gut microbiota remain poorly understood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!