2'-Fucosyllactose Promotes Colonization of and Prevents Colitis In Vitro and in Mice.

is a potential candidate for ulcerative colitis prevention. Considering that it utilizes 2'-fucosyllactose (2'FL) for growth, 2'FL can be used to enrich the abundance of in feces. However, whether the crosswalk between 2'FL and can promote the intestinal colonization of remains unclear. In this study, we explored the effect and the underlying mechanism of 2'FL on the colonization of in vitro and in vivo as well as its alleviating effect on colitis. Our results revealed that 2'FL can serve as a carbon source of to support the growth and increase cell-surface hydrophobicity and the expression of the genes coding fibronectin-binding autotransporter adhesin to promote the adhesion to Caco2/HT29 methotrexate (MTX) cells but not of galactooligosaccharides (GOS) and glucose. Moreover, 2'FL could increase the host mucin formation to promote the adhesion of to Caco2/HT29 MTX cells but not of GOS and glucose. Furthermore, 2'FL could significantly increase the colonization of in the gut to alleviate colitis in mice. Overall, the interplay between and 2'FL is expected to provide an advantageous ecological niche for so as to confer further health benefits against colitis.