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Native Mass Spectrometric Insights into the Formation and Stability of DNA Triplexes. | LitMetric

Native Mass Spectrometric Insights into the Formation and Stability of DNA Triplexes.

J Am Soc Mass Spectrom

Discipline of Chemistry, The University of Adelaide, Adelaide, South Australia 5005, Australia.

Published: March 2024

AI Article Synopsis

  • Deoxyribonucleic acid (DNA) is a crucial biomolecule that carries genetic information essential for the development and maintenance of living organisms, but higher order structures like DNA triplexes are not well understood.
  • Research indicates that DNA triplexes can form under normal physiological conditions, and native mass spectrometry is an effective method for exploring their formation and stability.
  • The study focuses on how various experimental factors, such as buffer salt concentration and pH, affect the detection of these triplex structures in mass spectrometry, providing insights for optimal testing conditions.

Article Abstract

Deoxyribonucleic acid is a genetic biomacromolecule that contains the inherited information required to build and maintain a living organism. While the canonical duplex DNA structure is rigorously characterized, the structure and function of higher order DNA structures such as DNA triplexes are comparatively poorly understood. Previous literature has shown that these triplexes can form under physiological conditions, and native mass spectrometry offers a useful platform to study their formation and stability. However, experimental conditions including buffer salt concentration, pH, and instrumentation parameters such as ion mode can confound analysis by impacting the amount of triplex observed by mass spectrometry. Using model 30mer Y-type triplex sequences, we demonstrate the influence a range of experimental variables have on the detection of DNA triplex structures, informing suitable conditions for the study. When carefully considered conditions are used, mass spectrometry offers a powerful complementary tool for the analysis of higher order DNA assemblies.

Download full-text PDF

Source
http://dx.doi.org/10.1021/jasms.3c00425DOI Listing

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