AI Article Synopsis

  • - The study investigates the role of N-methyladenosine (m6A) RNA methylation regulators in the development of bronchopulmonary dysplasia (BPD), a condition affecting premature infants, as its relationship with gene expression is not well understood.
  • - Researchers identified and compared differentially expressed m6A regulators in BPD patients through transcriptome data analysis, revealing significant changes in molecules like IGF2BP1/2/3 and classifying BPD into two subsets based on severity.
  • - Functional analysis indicated a disrupted immune-related signaling pathway in one subset of BPD, suggesting that understanding m6A regulation could offer new insights into diagnosing and treating this condition.

Article Abstract

N-methyladenosine (m6A) regulates gene expression and governs many important biological processes. However, the function of m6A in the development of bronchopulmonary dysplasia (BPD) remains poorly characterized. Thus, the purpose of this investigation was to evaluate the effects of m6A RNA methylation regulators on the development of BPD. BPD-related transcriptome data were downloaded from the GEO database. Differentially expressed m6A methylation regulators between BPD and control group were identified. Consensus clustering was conducted for the classification of BPD and association between clusters and BPD phenotypes were explored. Analysis of differentially expressed genes (DEGs) and immune-related DEGs was performed. The GSEA, GO and KEGG analyses were used to interpret the functional enrichments. The composition of immune cell subtypes in BPD subsets was predicted by CIBERSORT analysis. Compared with the control group, expression of most m6A regulators showed significant alteration, especially for IGF2BP1/2/3. BPD was classified into 2 subsets, and cluster 1 was correlated with severe BPD. Furthermore, the results of functional enrichment analyses showed a disturbed immune-related signaling pathway. Based on CIBERSORT analysis, we found that the proportion of immune cell subsets changed between cluster 1 and cluster 2. Our study revealed the implication of m6A methylation regulators in the development of BPD, which might provide a novel insight for the diagnosis and treatment of BPD.

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Source
http://dx.doi.org/10.1007/s10528-024-10664-1DOI Listing

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