Common bloom-forming cyanobacteria produce complex strain-specific mixtures of secondary metabolites. The beneficial and toxic properties of these metabolite mixtures have attracted both research and public health interest. The advancement of mass spectrometry-based platforms and metabolomics data processing has accelerated the identification of new metabolites and feature dereplication from microbial sources. The objective of this study was to use metabolomics data processing to decipher the intracellular cyanopeptide diversity of six strains collected from Canadian lakes. Data-dependent acquisition experiments were used to collect a non-targeted high-resolution mass spectrometry dataset. Principal component analysis and factor loadings were used to visualize cyanopeptide variation between strains and identified features contributing to the observed variation. GNPS molecular networking was subsequently used to show the diversity of cyanopeptides produced by the strains. Each strain produced a unique mixture of cyanopeptides, and a total of 225 cyanopeptides were detected. sp. CPCC 735 produced the most ( = 68) cyanopeptides, and CPCC 732 produced the fewest ( = 27). Microcystins and anabaenopeptins were detected from all strains. Cyanopeptolins, microviridins and aeruginosins were detected from five, four and two strains, respectively. Cyanopeptolin ( = 80) and anabaenopeptin ( = 61) diversity was the greatest, whereas microcystins ( = 21) were the least diverse. Interestingly, three of the strains had different cyanopeptide profiles, despite being collected from the same lake at the same time. This study highlights the diversity of cyanopeptides produced by and further hints at the underestimated cyanopeptide diversity from subpopulations of chemotypic cyanobacteria in freshwater lakes.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10891876PMC
http://dx.doi.org/10.3390/toxins16020110DOI Listing

Publication Analysis

Top Keywords

strains collected
8
collected canadian
8
canadian lakes
8
metabolomics data
8
data processing
8
cyanopeptide diversity
8
diversity cyanopeptides
8
cyanopeptides produced
8
detected strains
8
strains
7

Similar Publications

Clinical and molecular characteristics of KPC-producing Klebsiella pneumoniae bloodstream infections: results of a multicentre study.

J Glob Antimicrob Resist

January 2025

Infectious Disease Clinic, Dept. Of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Italy; Infectious Disease, Azienda Sanitaria Pesaro-Urbino, Pesaro, Italy.

Objectives: KPC-producing Klebsiella pneumoniae (KPC-Kp) is a great cause of concern and it is often associated with bloodstream infections (BSIs) and a high mortality rate. We identified the risk factors of KPC-Kp BSIs observed in three Italian hospitals and studied the epidemiology of KPC-Kp strains.

Methods: We performed a retrospective analysis of KPC-Kp BSIs from 2014 to 2019 in three hospitals in Central Italy (Ancona, Pesaro-Fano, and Perugia).

View Article and Find Full Text PDF

Genomic evolution of SARS-CoV-2 in Morocco: Insights from Whole Genome Sequences collected from 2020 to 2024.

Virus Res

January 2025

Molecular Biology and Functional Genomics Platform, National Centre for Scientific and Technical Research (CNRST), Rabat, Morocco; Genomic Centre for Human Pathologies (GENOPATH), Neuroscience and Neurogenetics Research Team, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco. Electronic address:

This study investigates the evolution and genetic diversity of SARS-CoV-2 strains circulating in Morocco to track the spread, clade distributions and mutations of the virus across various regions from February 2020 to June 2024. The genome sequences were retrieved from the GISAID database. A total of 2630 SARS-CoV-2 genome sequences were analyzed using bioinformatic tools such as Nextclade, followed by phylogenetic and statistical analyses.

View Article and Find Full Text PDF

Reptile trade and chelonians-associated Salmonellosis in humans: A public health concern.

Comp Immunol Microbiol Infect Dis

January 2025

Laboratory of Epidemio-surveillance, Health, Production & Reproduction, Cell Therapy of Domestic and Wild Animals, Department of Veterinary Sciences, Faculty of Nature and Life Sciences, University of Chadli Bendjedid, El Tarf 36000, Algeria.

Animal trade has become a serious criminal practice in the world. Every day thousands of exotic wild animals, including reptiles, are farmed and sold worldwide. The illegal collection of turtles and tortoises remains completely unsupervised and represents a big challenge for responsible authorities.

View Article and Find Full Text PDF

Next-generation sequencing analysis of bacterial species present in the sequestrum of medication-related osteonecrosis of the jaw patients.

Arch Oral Biol

January 2025

Department of Oral and Maxillofacial Surgery, Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, School of Dentistry, Pusan National University, Yangsan, South Korea; Dental Research Institute, Pusan National University Dental Hospital, Yangsan, South Korea. Electronic address:

Objective: Antiresorptives cases of adverse reactions to highly effective drugs used to treat skeletal-related events that occur during treatment of osteoporosis or malignancy have been reported in the jaw. To date, there is no clear pathophysiology for medication-related osteonecrosis of the jaw (MRONJ), but the role of inflammation or infection has been proposed as one of several possible pathogenesis theories. This study was designed to investigate the role of infection in the pathogenesis of MRONJ by analyzing the microbial communities observed in the bone samples from MRONJ patients.

View Article and Find Full Text PDF

Analysis of pathogen distribution, drug sensitivity and inflammatory indicators related to pelvic infection after hysterectomy.

Eur J Obstet Gynecol Reprod Biol

January 2025

Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu, PR China. Electronic address:

Objective: To analyse the distribution of pathogens, risk factors and inflammatory indicators related to pelvic infection after hysterectomy.

Methods: Patients who underwent hysterectomy at Women's Hospital of Nanjing Medical University between January 2022 and January 2023 were recruited into this study. Vaginal secretions from patients with suspected postoperative pelvic infection were collected for pathogen cultivation, identification, and antimicrobial susceptibility analysis.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!