AI Article Synopsis
- - The medium-chain dehydrogenase/reductase (MDR) superfamily consists of various members that are vital for growth, metabolism, and stress resistance in organisms, but their roles in certain contexts remain underexplored.
- - Using bioinformatics and RNA sequencing, the study analyzed the MDR superfamily's influence on fluconazole resistance, identifying 73 genes with NADPH-binding motifs and constructing a phylogenetic tree.
- - Findings revealed that fluconazole-resistant strains showed increased expression of xylitol dehydrogenase and reduced expression of alcohol and sorbitol dehydrogenases, indicating these strains are more adaptable to environmental changes and have a broader range of carbon source utilization.
Article Abstract
The medium-chain dehydrogenase/reductase (MDR) superfamily contains many members that are widely present in organisms and play important roles in growth, metabolism, and stress resistance but have not been studied in . In this study, bioinformatics and RNA sequencing methods were used to analyze the MDR superfamily of and its regulatory effect on fluconazole resistance. A phylogenetic tree was constructed using , , and and 73 were identified, all of which contained NADPH-binding motifs. contained 20 that were unevenly distributed across six chromosomes. () had similar 3D structures but varied greatly in their genetic evolution at different phylum levels. RNA-seq and gene expression analyses revealed that the fluconazole-resistant strain upregulates xylitol dehydrogenase, and downregulated alcohol dehydrogenase and sorbitol dehydrogenase concluded that the fluconazole-resistant strain was less selective toward carbon sources and had higher adaptability to the environment. Overall, our study contributes to our understanding of , providing a basis for further analysis of the genes associated with drug resistance in .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10889790 | PMC |
| http://dx.doi.org/10.3390/jof10020123 | DOI Listing |
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