This paper proposes the transformation S→C→, where is a digital gray-level image and C→ is a vector expressed through the textural space. The proposed transformation is denominated Vectorial Image Representation on the Texture Space (VIR-TS), given that the digital image is represented by the textural vector C→. This vector C→ contains all of the local texture characteristics in the image of interest, and the texture unit T→ entertains a vectorial character, since it is defined through the resolution of a homogeneous equation system. For the application of this transformation, a new classifier for multiple classes is proposed in the texture space, where the vector C→ is employed as a characteristics vector. To verify its efficiency, it was experimentally deployed for the recognition of digital images of tree barks, obtaining an effective performance. In these experiments, the parametric value λ employed to solve the homogeneous equation system does not affect the results of the image classification. The VIR-TS transform possesses potential applications in specific tasks, such as locating missing persons, and the analysis and classification of diagnostic and medical images.
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http://dx.doi.org/10.3390/jimaging10020048 | DOI Listing |
Adv Sci (Weinh)
January 2025
Translational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.
Viral vector and lipid nanoparticle based gene delivery have limitations around spatiotemporal control, transgene packaging size, and vector immune reactivity, compromising translation of nucleic acid (NA) therapeutics. In the emerging field of DNA and particularly RNA-based gene therapies, vector-free delivery platforms are identified as a key unmet need. Here, this work addresses these challenges through gene electrotransfer (GET) of "naked" polyanionic DNA/mRNA using a single needle form-factor which supports "electro-lens" based compression of the local electric field, and local control of tissue conductivity, enabling single capacitive discharge minimal charge gene delivery.
View Article and Find Full Text PDFRespiratory syncytial virus (RSV) remains the primary cause of lower respiratory tract infections, particularly in infants and the elderly. In this study, we employed reverse genetics to generate a chimeric influenza virus expressing neuraminidase-3F protein conjugate with three repeats of the RSV F protein protective epitope inserted into the NA gene of A/California/7/2009 ca (CA/AA ca), resulting in rFlu/RSV/NA-3F (hereafter, rFRN3). The expression of NA-3F protein was confirmed by Western blotting.
View Article and Find Full Text PDFHum Gene Ther
September 2023
Department of Medical Genetics, Center for Molecular Medicine and Therapeutics, BC Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada.
Lipoprotein lipase deficiency (LPLD) results from mutations within the gene that lead to a complete lack of catalytically active LPL protein. Glybera was one of the first adeno-associated virus (AAV) gene replacement therapy to receive European Medicines Agency regulatory approval for the treatment of LPLD. However, Glybera is no longer marketed potentially due to a combination of economical, manufacturing, and vector-related issues.
View Article and Find Full Text PDFNat Nanotechnol
October 2019
Department of Materials Science and Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Delivery technologies for the CRISPR-Cas9 (CRISPR, clustered regularly interspaced short palindromic repeats) gene editing system often require viral vectors, which pose safety concerns for therapeutic genome editing. Alternatively, cationic liposomal components or polymers can be used to encapsulate multiple CRISPR components into large particles (typically >100 nm diameter); however, such systems are limited by variability in the loading of the cargo. Here, we report the design of customizable synthetic nanoparticles for the delivery of Cas9 nuclease and a single-guide RNA (sgRNA) that enables the controlled stoichiometry of CRISPR components and limits the possible safety concerns in vivo.
View Article and Find Full Text PDFAm J Med Genet A
December 2011
State Key Laboratory of Oral Disease, West China College of Stomatology, Sichuan University, Chengdu, PR China.
The noncoding SNP rs7205289, located in the microRNA-140 gene has been associated with cleft palate risk. MiR-140 was found to regulate zebrafish palatal development in vivo and its expression level be reduced by environmental smoke exposure in vitro. Therefore, we sought to investigate whether the A allele of rs7205289 and maternal smoke exposure during the first trimester might contribute to cleft palate risk by regulating microRNA-140.
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