In this study, we examine the topography and adhesion images of the cell surface of neutrophils during the activation process. Our analysis of cell surface parameters indicates that the most significant changes in neutrophils occur within the first 30 min of activation, suggesting that reactive oxygen species may require approximately this amount of time to activate the cells. Interestingly, we observed surface granular structure as early as 10 min after neutrophil activation when examining atomic force microscopy images. This finding aligns with the reorganization observed within the cells under confocal laser scanning microscopy. By analyzing the cell surface images of adhesion, we identified three spatial surface parameters that correlate with the activation time. This finding enables us to estimate the degree of activation by using atomic force microscopy maps of the cell surface.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10886474PMC
http://dx.doi.org/10.3390/cells13040306DOI Listing

Publication Analysis

Top Keywords

cell surface
20
surface parameters
12
atomic force
12
force microscopy
12
neutrophil activation
8
activation
6
surface
6
cell
5
parameters accessing
4
accessing neutrophil
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!