[This corrects the article DOI: 10.1039/D2MD00186A.].
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http://dx.doi.org/10.1039/d4md90004a | DOI Listing |
Anal Chem
December 2024
Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario L8S 4M1, Canada.
Mass spectrometry (MS)-based metabolomics often rely on separation techniques when analyzing complex biological specimens to improve method resolution, metabolome coverage, quantitative performance, and/or unknown identification. However, low sample throughput and complicated data preprocessing procedures remain major barriers to affordable metabolomic studies that are scalable to large populations. Herein, we introduce PeakMeister as a new software tool in the R statistical environment to enable standardized processing of serum metabolomic data acquired by multisegment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS), a high-throughput separation platform (<4 min/sample) which takes advantage of a serial injection format of 13 samples within a single analytical run.
View Article and Find Full Text PDFJ Cheminform
December 2024
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, 77900, Olomouc, Czech Republic.
Background: Cell-free DNA (cfDNA), a broadly applicable biomarker commonly sourced from urine or blood, is extensively used for research and diagnostic applications. In various settings, genetic and epigenetic information is derived from cfDNA. However, a unified framework for its processing is lacking, limiting the universal application of innovative analysis strategies and the joining of data sets.
View Article and Find Full Text PDFCommun Biol
December 2024
College of Biology, Hunan University, Changsha, China.
Error self-correction is crucial for analyzing long-read sequencing data, but existing methods often struggle with noisy data or are tailored to technologies like PacBio HiFi. There is a gap in methods optimized for Nanopore R10 simplex reads, which typically have error rates below 2%. We introduce DeChat, a novel approach designed specifically for these reads.
View Article and Find Full Text PDFAutophagy
December 2024
Peripheral Neuropathy Research Group, Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.
HSPB1 [heat shock protein family B (small) member 1] and HSPB8 are essential molecular chaperones for neuronal proteostasis, as they prevent protein aggregation. Mutant HSPB1 and HSPB8 primarily harm peripheral neurons, resulting in axonal Charcot-Marie-Tooth neuropathies (CMT2). Macroautophagy/autophagy is a shared mechanism by which HSPB1 and HSPB8 mutations cause neuronal dysfunction.
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