[Effect of Knocking Out Gene by CRISPR-Cas9-Mediated Gene Editing Technique on Proliferation of Acute Myeloid Leukemia Cells].

Objective: To construct a acute myeloid leukemia (AML) cell line in which gene is stably knocked out by CRISPR-Cas9-mediated gene editing technique, so as to clarify the effect of gene knockout on the proliferation of AML cells, and preliminarily explore the role of gene in the pathogenesis of AML.

Methods: The expression of HOXA5 in bone marrow mononuclear cells (BMMC) of non-tumor hematological patients and newly diagnosed AML patients was detected by quantitative real-time PCR (qRT-PCR) and Western blot, respectively. The AML cell line KO-HOXA5-THP-1 was constructed in which gene was knocked out by CRISPR-Cas9-Mediated gene editing technique, and the knockout of HOXA5 gene was verified by qRT-PCR and Western blot, and the cell proliferation was detected by CCK-8 assay.

Results: Compared with non-tumor hematological patients, the levels of gene and protein in BMMC of newly diagnosed AML patients were significantly increased ( <0.05). The stable knockout cell line can be obtained by CRISPR-Cas9-Mediated gene editing technique, and the proliferation ability of THP-1 cells with gene knockout was significantly decreased ( <0.05).

Conclusion: is highly expressed in AML cells, and knocking out can significantly affect the proliferation ability of AML cells, which provides a new potential therapeutic target for the precise treatment of AML.