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Neuronal reprogramming of mouse and human fibroblasts using transcription factors involved in suprachiasmatic nucleus development. | LitMetric

AI Article Synopsis

  • - The suprachiasmatic nucleus (SCN) in the hypothalamus regulates circadian rhythms and is made up of diverse neurons that express specific signaling peptides like VIP and AVP.
  • - Researchers enhanced a neuronal conversion method by adding transcription factors (TFs) crucial for SCN neurogenesis, successfully converting mouse and human fibroblasts into neurons.
  • - The study highlights the significant role of these TFs in developing VIP-expressing neurons in the SCN, indicating potential for future regeneration and treatment of related diseases.

Article Abstract

The hypothalamic suprachiasmatic nucleus (SCN) is composed of heterogenous populations of neurons that express signaling peptides such as vasoactive intestinal polypeptide (VIP) and arginine vasopressin (AVP) and regulate circadian rhythms in behavior and physiology. SCN neurons acquire functional and morphological specializations from waves of transcription factors (TFs) that are expressed during neurogenesis. However, the generation of SCN neurons has never been achieved. Here we supplemented a highly efficient neuronal conversion protocol with TFs that are expressed during SCN neurogenesis, namely , , , and . Neurons induced from mouse and human fibroblasts predominantly exhibited neuronal properties such as bipolar or multipolar morphologies, GABAergic neurons with expression of VIP. Our study reveals a critical contribution of these TFs to the development of vasoactive intestinal peptide (Vip) expressing neurons in the SCN, suggesting the regenerative potential of neuronal subtypes contained in the SCN for future SCN regeneration and disease remodeling.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10879699PMC
http://dx.doi.org/10.1016/j.isci.2024.109051DOI Listing

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