Decoy oligodeoxynucleotides (ODNs) allow targeting undruggable transcription factors, such as STAT3, but their limited potency and lack of delivery methods hampered translation. To overcome these challenges, we conjugated a STAT3-specific decoy to thalidomide, a ligand to cereblon in E3 ubiquitin ligase complex, to generate a proteolysis-targeting chimera (STAT3D). STAT3D downregulated STAT3 in target cells, but not STAT1 or STAT5. Computational modeling of the STAT3D ternary complex predicted two surface lysines, K601 and K626, in STAT3 as potential ubiquitination sites. Accordingly, K601/K626 point mutations in STAT3, as well as proteasome inhibition or cereblon deletion, alleviated STAT3D effect. Next, we conjugated STAT3D to a CpG oligonucleotide targeting Toll-like receptor 9 (TLR9) to generate myeloid/B cell-selective C-STAT3D. Naked C-STAT3D was spontaneously internalized by TLR9 myeloid cells, B cells, and human and mouse lymphoma cells but not by T cells. C-STAT3D effectively decreased STAT3 protein levels and also STAT3-regulated target genes critical for lymphoma cell proliferation and/or survival (, , and ). Finally, local C-STAT3D administration to human Ly3 lymphoma-bearing mice triggered tumor regression, while control C-STAT3D and C-SCR treatments had limited effects. Our results underscore the feasibility of using a PROTAC strategy for cell-selective, decoy oligonucleotide-based STAT3 targeting of and potentially other tumorigenic transcription factors for cancer therapy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10879796 | PMC |
http://dx.doi.org/10.1016/j.omtn.2024.102137 | DOI Listing |
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