AI Article Synopsis
- - The study addresses challenges in volumetric imaging of living organisms, focusing on the need for high speed and resolution across a large field of view to observe dynamic processes in 3D at cellular levels.
- - A new microscopy technique called mesoscopic oblique plane microscopy (Meso-OPM) was developed, utilizing a diffractive light sheet to enhance axial resolution by sixfold compared to existing methods.
- - Meso-OPM achieved a field of view of up to 5.4 mm × 3.3 mm and a resolution of 2.5 μm × 3 μm × 6 μm, allowing for comprehensive imaging of zebrafish larvae, including neuronal activity and blood flow dynamics
Article Abstract
Fundamental understanding of large-scale dynamic connectivity within a living organism requires volumetric imaging over a large field of view (FOV) at biologically relevant speed and resolution. However, most microscopy methods make trade-offs between FOV and axial resolution, making it challenging to observe highly dynamic processes at cellular resolution in 3D across mesoscopic scales (e.g., whole zebrafish larva). To overcome this limitation, we have developed mesoscopic oblique plane microscopy (Meso-OPM) with a diffractive light sheet. By augmenting the illumination angle of the light sheet with a transmission grating, we improved the axial resolution approximately sixfold over existing methods and approximately twofold beyond the diffraction limitation of the primary objective lens. We demonstrated a FOV up to 5.4 mm × 3.3 mm with resolution of 2.5 μm × 3 μm × 6 μm, allowing volumetric imaging of 3D cellular structures with a single scan. Applying Meso-OPM for imaging of zebrafish larvae, we report here whole-body volumetric recordings of neuronal activity at 2 Hz volume rate and whole-body volumetric recordings of blood flow dynamics at 5 Hz with 3D cellular resolution.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10881189 | PMC |
| http://dx.doi.org/10.1364/optica.471101 | DOI Listing |
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