AI Article Synopsis
- - The study assessed the cytotoxicity and genotoxicity of calcium-silicate based sealers, comparing them to a conventional epoxy-based sealant using two cell lines: gingival fibroblasts (hGF) and a monocyte/macrophage line (SC).
- - Results showed that BioRoot Flow significantly reduced viability of hGF cells after both 24 and 48 hours, while the AH Plus Bioceramic Sealer improved SC cell viability after 24 hours.
- - Both BioRoot Flow and BioRoot RCS exhibited potential genotoxic effects, causing DNA damage and apoptosis in hGF cells, which may lead to irritation and delayed healing in periapical tissues.
Article Abstract
The aim of this study was to evaluate cytotoxicity and genotoxicity of calcium-silicate based sealers and comparing them with a gold standard-an epoxy-based sealant. Two experimental cell lines were used, gingival fibroblasts (hGF) and monocyte/macrophage peripheral blood cell line (SC). The cytotoxicity (XTT assay) and genotoxicity (comet assay) were evaluated both after 24-h and 48-h incubation. Additionally, after 48-h incubation, the cell apoptosis and cell cycle progression was detected. BioRoot Flow induced a significant decrease in hGF cells viability compared to the negative control groups both after 24-h (p < 0.001) and 48-h incubation (p < 0.01). In group with SC cells, after 24-h incubation significant increase in cells viability was detected for AH Plus Bioceramic Sealer in comparison to negative control (p < 0.05). BioRoot Flow and BioRoot RCS can be considered potentially genotoxic for the hGF cells after 48-h incubation (> 20% DNA damage). BioRoot Flow and BioRoot RCS, may have potential genotoxic effects and induce apoptosis in hGF cells which may irritate periapical tissues, resulting in a delayed healing. The findings of the study would be useful in selection of an appropriate sealant for root canal filling without causing cytotoxicity and genotoxicity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10876547 | PMC |
| http://dx.doi.org/10.1038/s41598-024-54726-1 | DOI Listing |
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