AI Article Synopsis
- Tobacco streak virus (TSV) poses a significant threat to various crops, yet there is a lack of infectious clones for studying its genetic properties.
- Researchers sequenced the full genome of a TSV-CNB isolate and created infectious cDNA clones for reverse genetics studies, utilizing yeast homologous recombination techniques.
- The study found that TSV-CNB can infect 10 different plant species across 5 families, indicating its broad host range, which will aid future research on virus interactions and effects on plants.
Article Abstract
Tobacco streak virus induces severe diseases on a wide range of plants and becomes an emerging threat to crop yields. However, the infectious clones of TSV remain to be developed for reverse genetics studies. Here, we obtained the full genome sequence of a TSV-CNB isolate and analyzed the phylogenetic characteristics. Subsequently, we developed the full-length infectious cDNA clones of TSV-CNB driven by 35 S promoter using yeast homologous recombination. Furthermore, the host range of TSV-CNB isolate was determined by Agrobacterium infiltration and mechanical inoculation. The results reveal that TSV-CNB can infect 10 plant species in 5 families including Glycine max, Vigna radiate, Lactuca sativa var. Ramosa, Dahlia pinnate, E. purpurea, Calendula officinalis, Helianthus annuus, Nicotiana. Benthamiana, Nicotiana tabacum and Chenopodium quinoa. Taken together, the TSV infectious clones will be a useful tool for future studies on viral pathogenesis and host-virus interactions.
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| http://dx.doi.org/10.1016/j.virol.2024.110013 | DOI Listing |
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