Psychromicrobium Xiongbiense sp. nov., an Actinobacterium Isolated from Forest Soil.

A novel Gram-stain-positive, oval-shaped, and non-flagellated bacterial strain YIM S02556 was isolated from forest soil in Xiongbi Town, Shizong County, Qujing City, Yunnan Province, southwestern China. The strain exhibited high pairwise 16 S rRNA gene sequence similarity with Psychromicrobium lacuslunae (97.3%) and Psychromicrobium silvestre (96.3%). Strain YIM S02556 exhibited an average nucleotide identity (ANI) of 72.5% with P. lacuslunae IHBB 11,108 and 72.8% ANI with P. silvestre AK 20-18. The digital DNA-DNA hybridization (dDDH) value between strain YIM S02556 and P. lacuslunae IHBB 11,108 was 20.2%, while with P. silvestre AK 20-18, the dDDH value was 20.8%. Strain YIM S02556 exhibited optimal growth at 28 °C, pH 7.0, without NaCl. Growth occurred within 10-37 ℃, pH 5.0-8.0, and in the presence of up to 5% w/v NaCl concentration. The genome size was 3.1 Mbp with 64.2% G + C content. The predominant menaquinone was MK-8(H). The major cellular fatty acid was anteiso-C. Based on the polyphasic analysis, strain YIM S02556 (= KCTC 49,805 = CCTCC AB2020166) represents a novel Psychromicrobium species in which the name Psychromicrobium xiongbiense sp.nov. was proposed.