AI Article Synopsis
- Fluorescent biosensors have transformed biomedical research by allowing real-time measurement of signaling in living cells, but they currently struggle with resolution and dimensionality.
- The introduction of newly engineered chemigenetic kinase activity biosensors utilizes the HaloTag7 protein with bright far-red synthetic fluorophores for improved imaging.
- This new technology supports advanced techniques like five-color multiplexing, 4D activity imaging, and super-resolution imaging using stimulated emission depletion microscopy.
Article Abstract
Fluorescent biosensors revolutionized biomedical science by enabling the direct measurement of signaling activities in living cells, yet the current technology is limited in resolution and dimensionality. Here, we introduce highly sensitive chemigenetic kinase activity biosensors that combine the genetically encodable self-labeling protein tag HaloTag7 with bright far-red-emitting synthetic fluorophores. This technology enables five-color biosensor multiplexing, 4D activity imaging, and functional super-resolution imaging via stimulated emission depletion (STED) microscopy.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10871310 | PMC |
| http://dx.doi.org/10.1101/2024.02.10.579766 | DOI Listing |
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