AI Article Synopsis
- Peroxynitrite (ONOO) is a significant reactive oxygen species (ROS) that can cause lipid peroxidation, leading to ferroptotic cell death, making its rapid detection essential for research.
- Researchers developed fluorescent probes (Rh-1, Rh-2, Rh-3) that react with ONOO, producing a strong fluorescent signal at 561 nm, with Rh-3 showing the best sensitivity and selectivity.
- Bioimaging with Rh-3 revealed that ferroptosis, triggered by erastin, increases levels of endogenous ONOO, while treatments with ferrostatin-1 and vitamin E effectively reduce ONOO production in live cells.
Article Abstract
Peroxynitrite (ONOO) is a critical ROS in living systems, and could induce lipid peroxidation which is the driver of ferroptotic cell death. Therefore, precise and rapid detection of cellular ONOO is critical for the deep study of the biological functions of ONOO during ferroptosis. Herein, we developed fluorescent probes (Rh-1, Rh-2 and Rh-3) for the rapid detection of intracellular ONOO during ferroptosis. These probes used bishydrazide groups as the reactive sites for ONOO. The response of these probes to ONOO resulted in the production of the emissive xanthene fluorophore, providing a marked enhancement in the fluorescence intensity at 561 nm. The probe Rh-3 exhibited prominent selectivity and sensitivity towards ONOO. Bioimaging experiments suggested that Rh-3 could be applied to image exogenous and endogenous ONOO in living cells. By fluorescence imaging, it was demonstrated that erastin-induced ferroptosis caused increased levels of the endogenous ONOO, and ferrostatin-1 (Fer-1) and vitamin E (VE) could markedly inhibit the excessive production of ONOO during ferroptosis in living cells.
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Article Synopsis
- Over-consumption of drugs can lead to drug-induced liver injury (DILI), and ferroptosis—a process influenced by reactive oxygen species—plays a crucial role in its development, marked by increasing levels of peroxynitrite (ONOO).
- A new fluorescence probe, DILI-ONOO, was created to detect and visualize ONOO in DILI, demonstrating high sensitivity and low cytotoxicity, and successfully illustrating changes in ONOO levels in lab models.
- This probe provides a novel approach for early diagnosis of DILI, indicating a relationship between ferroptosis and liver damage, and offers potential for evaluating treatment effectiveness.
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