Growth performance, serum parameters, inflammatory responses, intestinal morphology and microbiota of weaned piglets fed 18% crude protein diets with different ratios of standardized ileal digestible isoleucine to lysine.

The present study was to explore the Ile requirement of piglets fed 18% crude protein (CP) diets. Two hundred and fifty 28-day-old Duroc × Landrace × Yorkshire piglets (8.37 ± 1.92 kg) were randomly divided into 5 dietary treatments (10 piglets per replicate, 5 barrows and 5 gilts per replicate) with 45%, 50%, 55%, 60%, 65% standardized ileal digestible (SID) Ile-to-Lys ratios, and the SID Lys was formulated to 1.19%. The experimental design consisted of two phases (d 1 to 14 and d 15 to 28). Results showed that average daily gain (ADG) had a tendency to quadratically increase as the SID Ile-to-Lys ratio increased ( = 0.09), and the optimum SID Ile-to-Lys ratios required to maximize ADG were 48.33% and 54.63% for broken-line linear model and quadratic polynomial model, respectively. Different SID Ile-to-Lys ratios had no significant effects on average daily feed intake and gain-to-feed ratio. Dry matter ( < 0.01), CP ( = 0.01), ether extract ( = 0.04), gross energy ( < 0.01) and organic matter ( < 0.01) digestibility increased quadratically. Serum total cholesterol levels decreased linearly ( = 0.01) and quadratically ( < 0.01); aspartate aminotransferase ( < 0.01), interleukin-1β ( = 0.01), and tumor necrosis factor-α ( < 0.01) levels decreased quadratically; immunoglobulin G ( = 0.03) and immunoglobulin M ( = 0.01) concentrations increased quadratically. Serum Ser levels decreased linearly ( < 0.01) and quadratically ( = 0.01); Glu ( = 0.02), Arg ( = 0.05), and Thr ( = 0.03) levels decreased quadratically; Gly ( < 0.01) and Leu ( = 0.01) levels decreased linearly; Ile ( < 0.01) concentration increased linearly. Duodenal villus height ( < 0.01) and villus height to crypt depth ratio ( < 0.01) increased quadratically. The deficiency or excess of Ile decreased short chain fatty acid-producing bacteria abundance and increased pathogenic bacteria abundance. Overall, taking ADG as the effect index, the optimum SID Ile-to-Lys ratios of piglets offered 18% CP diets were 48.33% and 54.63% based on two different statistical models, respectively, and the deficiency or excess of lle negatively affected piglet growth rates and health status.