Clonal hematopoiesis (CH) is an independent risk factor for atherosclerotic cardiovascular disease. Murine models of CH suggest a central role of inflammasomes and IL-1β in accelerated atherosclerosis and plaque destabilization. Here we show using single-cell RNA sequencing in human carotid plaques that inflammasome components are enriched in macrophages, while the receptor for IL-1β is enriched in fibroblasts and smooth muscle cells (SMCs). To address the role of inflammatory crosstalk in features of plaque destabilization, we conducted SMC fate mapping in mice modeling or CH treated with IL-1β antibodies. Unexpectedly, this treatment minimally affected SMC differentiation, leading instead to a prominent expansion of fibroblast-like cells. Depletion of fibroblasts from mice treated with IL-1β antibody resulted in thinner fibrous caps. Conversely, genetic inactivation of during plaque regression promoted fibroblast accumulation and fibrous cap thickening. Our studies suggest that suppression of inflammasomes promotes plaque stabilization by recruiting fibroblast-like cells to the fibrous cap.
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http://dx.doi.org/10.1038/s44161-023-00405-9 | DOI Listing |
Tissue Cell
December 2024
Department of Anatomy and Embryology, Faculty of Medicine, Universidad Complutense de Madrid (UCM), Madrid, Spain; UCM Research Group No. 920202, Faculty of Medicine, Universidad Complutense de Madrid (UCM), Madrid, Spain.
Background: In recent years, the fasciae of the human body have received significant attention because of their crucial role in the transmission of muscle force. However, studies on the development of the fasciae, particularly the cervical fascia, remain scarce.
Purpose: This study was performed to examine the development of the fascia of the infrahyoid strap muscles, also known as the middle layer of the deep cervical fascia (MDCF), in 17 human embryos aged 6-8 weeks and 20 human foetuses aged 9-14 weeks.
Cell Death Dis
December 2024
Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, PR China.
Rheumatoid arthritis (RA) is a chronic autoimmune disorder marked by pain, inflammation, and discomfort in the synovial joints. It is critical to understand the pathological mechanisms of RA progression. MicroRNA-378 (miR-378) is highly expressed in the synovium of RA patients and positively correlated with disease severity, but its function and underlying mechanisms remain poorly understood.
View Article and Find Full Text PDFCell Biochem Funct
December 2024
Department of Rheumatology and Immunology, The First Affiliated Hospital at Xinjiang Medical University, Urumqi, People's Republic of China.
Rheumatoid arthritis (RA), a highly disabling autoimmune disease, is characterized by joint damage and synovial hyperplasia. This paper was designed to explore the effect and mechanisms of Aconitum diphtheria on the proliferation and apoptosis of RA fibroblast-like synoviocytes (RA-FLS). First, RA-FLS was treated with different doses of A.
View Article and Find Full Text PDFJ Thorac Dis
November 2024
Chongqing Medical University, Chongqing, the Department of Geriatrics, Chongqing General Hospital, Chongqing University, Chongqing, China.
Background: Rheumatoid arthritis-associated interstitial lung disease (RA-ILD) is a serious pulmonary complication in rheumatoid arthritis (RA) patients, is one of the leading causes of death in RA patients. This study was designed to determine whether pirfenidone and nintedanib can alleviate joint inflammation and pulmonary fibrosis in a mouse model of RA-ILD.
Methods: Male DBA/1 mice were injected with bovine type II collagen (bCII) to establish the RA-ILD model.
Mod Rheumatol
December 2024
Division of Rheumatology, Department of Medicine, Showa University School of Medicine, Tokyo, Japan.
Objectives A disintegrin and metalloproteinase (ADAM)-15 and vascular endothelial (VE)-cadherin are involved in angiogenesis. We investigated the relationship between ADAM-15 and VE-cadherin expressions in rheumatoid arthritis (RA). Methods VE-cadherin concentrations in the serum of patients with RA were measured using the enzyme linked immunosorbent assay (ELISA).
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