In the current study, a novel methylene blue (MB)-based colorimetric method for a quick, inexpensive, and facile approach for the determination of fouling intensity of reverse osmosis (RO) membrane has been reported. This technique is based on the interaction of MB with the organic foulants and shows the corresponding change in the colour intensity depending on the severity of fouling. The organic foulants, such as albumin, sodium alginate, and carboxymethyl cellulose (CMC), were chosen as model foulants, and the membranes were subjected to foul under extreme fouling conditions. The fouled membranes underwent an MB treatment followed by image-processing analyses. The severity of surface fouling of membranes was evaluated in terms of fouling intensity and correlated with the corresponding decline of permeate flux. The maximum fouling intensity of the albumin, sodium alginate, and CMC sodium were found to be 8.83, 23.38, and 9.19%, respectively, for the definite concentration of foulants. The physico-chemical interactions of the given foulants and MB were confirmed by changes in zeta potentials and increased sizes of the foulant by the dynamic light scattering technique. The surface fouling over the membrane surface was confirmed by the characterization of membranes.
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