AI Article Synopsis
- A thermophilic bacterium, Geobacillus kaustophilus HTA426, can degrade alkanes despite lacking specific alkane oxygenating enzyme genes.
- Researchers identified a gene called GkR2loxI (GK2771) that encodes a novel enzyme functioning as an alkane monooxygenase/hydroxylase.
- This discovery enhances our understanding of the R2lox protein family and reveals new evolutionary insights into bacterial alkane metabolism.
Article Abstract
We have accidentally found that a thermophilic Geobacillus kaustophilus HTA426 is capable of degrading alkanes although it has no alkane oxygenating enzyme genes. Our experimental results revealed that a putative ribonucleotide reductase small subunit GkR2loxI (GK2771) gene encodes a novel heterodinuclear Mn-Fe alkane monooxygenase/hydroxylase. GkR2loxI protein can perform two-electron oxidations similar to homonuclear diiron bacterial multicomponent soluble methane monooxygenases. This finding not only answers a long-standing question about the substrate of the R2lox protein clade, but also expands our understanding of the vast diversity and new evolutionary lineage of the bacterial alkane monooxygenase/hydroxylase family.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10867098 | PMC |
| http://dx.doi.org/10.1007/s00792-024-01332-8 | DOI Listing |
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