AI Article Synopsis
- The study aimed to find target genes for detecting Orientia tsutsugamushi (OT) in cases of pediatric acute encephalitis syndrome (pAES).
- DNA was extracted from blood samples of 100 pAES patients, testing for several genes including a newly identified 27 kDa gene.
- The 56 kDa gene was found to be the most effective for OT detection, successfully identifying OT in 41.4% of IgM-positive and 64.4% of IgM-negative cases, while other gene tests were ineffective.
- Implementing tests for the 56 kDa gene alongside serological methods is recommended for better detection in areas where OT is common.
Article Abstract
Objectives: To identify the potential target genes for detection of Orientia tsutsugamushi (OT) in pediatric acute encephalitis syndrome (pAES).
Methods: DNA was extracted from whole blood of 100 pAES cases having tested positive (n = 41) and negative (n = 59) for scrub typhus (ST) by IgM ELISA. These samples were subjected to standard PCR for 56 kDa, 47 kDa, 16 s rRNA, groEL, traD genes and the newly identified 27 kDa gene.
Results: Among the selected gene targets, 56 kDa demonstrated its superiority for OT detection over the other tested genes. The presence of OT was confirmed via PCR targeting 56 kDa gene in 17 out of the 41 (41.4 %) IgM-positive ST AES cases and 38 out of the 59 (64.4 %) ST IgM negative cases. None of the other gene targets were amplified.
Conclusion: Integration of serological diagnosis with molecular diagnostics targeting the 56 kDa gene for routine testing of AES patients would facilitate detection of OT in AES endemic regions.
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| http://dx.doi.org/10.1016/j.idnow.2024.104865 | DOI Listing |
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