AI Article Synopsis
- The study aimed to establish reference ranges for T cell proliferation induced by phytohemagglutinin (PHA-SI) and Bacillus Calmette-Guérin (BCG-SI) to assist in clinical diagnosis.
- Researchers evaluated data from 359 healthy children and 35 patients with cellular immunodeficiency using a colorimetric method to measure cell proliferation and determined reference ranges at the 2.5th and 97.5th percentiles.
- The results showed significant differences in proliferation between healthy controls and patients with immunodeficiency, providing crucial thresholds for diagnosing congenital immunodeficiency diseases.
Article Abstract
Purpose: To establish reference ranges (RRs) for stimulation index of T cell proliferation triggered by phytohemagglutinin (PHA-SI) and Bacillus Calmette-Guérin (BCG-SI).
Methods: This study investigated data from 359 healthy children and 35 patients with cellular immunodeficiency as positive controls (2010-2021). We applied a colorimetric-based method (BrdU) to measure proliferation and determine the RRs at the 2.5th and 97.5th percentiles (95% confidence intervals). A cross-validation approach was performed.
Results: In healthy controls, the RRs for PHA-SI and BCG-SI ranged between 3 and 5.2 and 2.52 to 5.2, respectively. PHA-SI and BCG-SI were in Severe Combined Immunodeficiency (SCID) patients from 1.2 to 2.5 and 0 to 2, while in Mendelian susceptibility to mycobacterial diseases (MSMD) patients, 2.53 to 4.5 and 0.74 to 2.2, respectively. The thresholds' accuracy was checked for testing reference intervals with diagnostic effects.
Conclusion: This study establishes PHA-SI and BCG-SI reference ranges to aid in diagnosing and treating congenital immunodeficiency diseases.
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| http://dx.doi.org/10.1016/j.clim.2024.109937 | DOI Listing |
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