Background: The adhesion genes are responsible for biofilm production which leads to chronic diseases like urinary tract infections (UTIs). Uropathogenic Escherichia coli (UPEC) is the most predominant pathogen involved in UTIs. This study aims to evaluate the relationship between adhesion genes and bacterial biofilm that form by UPEC.
Methods: Fifty clinical isolates of E. coli from patients infected with UTIs were identified and antimicrobial resistance was tested by MIC assay. A polymerase chain reaction (PCR), a quick and sensitive assay to identify the adhesions operon (Afa, papG, flu, and fimH), was developed using eight primers and used for amplification. E. coli K-12 strain and E. coli J96 were used as a negative and a positive control for detection of adhesion genes.
Results: The study reported 70% of isolates produce strong biofilm. Adhesion genes showed as follow Afa (64% n = 33), papG (42% n = 23), flu (94% n = 52), fimH (86% n = 45).
Conclusions: The resistance to non-Beta lactam antibiotic was significantly correlated with the availability of genes that encode for adhesion. These genes were highly correlated to biofilm formation in E. coli clinical isolates.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.7754/Clin.Lab.2023.230525 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
miRNA Expression Profile in Primary Limbal Epithelial Cells of Aniridia Patients.
Invest Ophthalmol Vis Sci
January 2025
Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Congenital Aniridia Research, Homburg/Saar, Germany, Saarland University, Homburg/Saar, Germany.
Purpose: This study evaluates the microRNA (miRNA) expression profile in primary limbal epithelial cells (pLECs) of patients with aniridia.
Methods: Primary human LECs were sampled and isolated from 10 patients with aniridia and 10 healthy donors. The miRNA profile was analyzed using miRNA microarrays.
Single-Cell RNA-seq Reveals Increased and Activated Post-Capillary Venule Endothelial Cells in Erythrodermic Psoriasis.
Inflammation
January 2025
Department of Dermatology, The University of Hong Kong-Shenzhen Hospital, Guangdong Province, Shenzhen, People's Republic of China.
Erythrodermic psoriasis (EP) is a life-threatening variant of psoriasis. In this study, we contrasted the vascular endothelial cells (ECs) in EP lesions against those in psoriasis vulgaris and healthy controls. Utilizing single-cell RNA sequencing, immunofluorescence, and flow cytometry on human and mouse samples, we observed a marked increase and activation of EP ECs, which upregulated genes relative to angiogenesis, leukocyte adhesion and antigen presentation.
View Article and Find Full Text PDFBackground: Alzheimer's Disease (AD) proteomic studies have focused on understanding the pathophysiology of AD during the late stages of AD. However, recent studies have suggested that the preclinical stage of AD represents a golden window for intervention. Yet, little is known about the influence of the cerebrospinal fluid (CSF) molecular environment on the mechanisms underlying the pathogenesis of AD during the preclinical stage.
View Article and Find Full Text PDFDeveloping Topics.
Alzheimers Dement
December 2024
Oregon Health & Science University, Portland, OR, USA.
Background: Alzheimer's disease (AD) is the fifth leading cause of death for individuals aged 65 and older. Since FDA approved therapies have limited efficacy, there is a need to develop new treatments based on a better understand of pathological changes occurring in tissues from humans with AD. Central nervous system cells utilize extracellular vesicles (EVs) to package and secrete miRNAs where they mediate cell-to-cell communication.
View Article and Find Full Text PDFDeveloping Topics.
Alzheimers Dement
December 2024
Rush Alzheimer's Disease Center, Rush University Medical Center, Chicago, IL, USA.
Background: The choroid plexus (ChP) is formed by epithelial cells and stromal fibroblasts which act as a blood-cerebrospinal fluid (CSF) barrier, play a key role in maintaining brain homeostasis, and provide a niche for immune cells. ChP dysfunction has been implicated in Alzheimer's Disease (AD), including changes in CSF secretion, increased apoptosis, and dysregulated immune, mitochondrial, and transporter functions.
Method: Here, we performed single-nuclei RNA-Sequencing (snRNA-Seq) on 965,647 ChP nuclei from 68 ROSMAP participants with no cognitive impairment (NCI), mild cognitive impairment (MCI) or Alzheimer's Dementia (ADem).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!