Int J Mol Sci
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy.
Published: February 2024
Probiotic therapy needs consideration as an alternative strategy to prevent and possibly treat corneal infection. This study aimed to assess the preventive effect of and subsp. on reducing the infection of human corneal epithelial (HCE) cells caused by . The probiotics' preventive effect against infection was evaluated in cell monolayers pretreated with each probiotic 1 h and 24 h prior to challenge followed by 1 h and 24 h of growth in combination. Cell adhesion, cytotoxicity, anti-inflammatory, and antinitrosative activities were evaluated. and adhered to HCE cells, preserved occludin tight junctions' integrity, and increased mucin production on a SkinEthic HCE model. Pretreatment with or significantly protected HCE cells from infection at 24 h, increasing cell viability at 110% (110.51 ± 5.15; ≤ 0.05) and 137% (137.55 ± 11.97; ≤ 0.05), respectively. Each probiotic showed anti-inflammatory and antinitrosative activities, reducing TNF-α level ( ≤ 0.001) and NO amount ( ≤ 0.001) and reestablishing IL-10 level ( ≤ 0.001). In conclusion, this study demonstrated that and exert protective effects in the context of corneal infection caused by by restoring cell viability and modulating inflammatory cytokine release.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10855269 | PMC |
http://dx.doi.org/10.3390/ijms25031770 | DOI Listing |
Invest Ophthalmol Vis Sci
December 2024
State Key Laboratory of Ophthalmology, Optometry and Vision Science, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Purpose: The purpose of this study was to investigate the potential roles of endoplasmic reticulum (ER) stress in the development of dry eye disease (DED).
Methods: Single-cell RNA sequencing (scRNA-seq) data from the Gene Expression Omnibus (GEO) database, derived from corneal tissues of a dry eye mouse model, was processed using the Seurat R program. The results were validated using a scopolamine-induced dry eye mouse model and a hyperosmotic-induced cell model involving primary human corneal epithelial cells (HCECs) and immortalized human corneal epithelial (HCE-2) cells.
Exp Eye Res
December 2024
Aravind Medical Research Foundation, Madurai, Tamil Nadu, India. Electronic address:
The need for better and simpler alternative crosslinking strategies to treat keratoconus (KC) is becoming essential as there is only a single approved way to treat it. Recently, conventional UV-A Riboflavin crosslinking is proven to have some disadvantages such as causing damage to the corneal endothelium and inducing keratocyte apoptosis. A chemical cross-linker (CXL) using carbodiimide chemistry and an octanedioic acid spacer is found effective in stiffening the cornea and has the potential to be developed as an alternative therapy to halt KC progression.
View Article and Find Full Text PDFBiochem Biophys Res Commun
January 2025
Institute of Biomedical Engineering, College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, China. Electronic address:
Keratoconus (KC) is an ectatic cornea disease with high prevalence and asymptomatic at early stage, leading to decreased visual acuity and even cornea transplantation. However, the etiology mechanism of keratoconus is still poorly understood. Oxidative stress (OS) and extracellular matrix (ECM) remodeling play critical roles in keratoconus development.
View Article and Find Full Text PDFCarbohydr Polym
February 2025
CNR - Istituto di Chimica Biomolecolare, Via Paolo Gaifami 18, I-95126 Catania, Italy. Electronic address:
Biomed Pharmacother
December 2024
Translational Complementary Medicine, Department of Pharmaceutical Sciences, University of Basel, Switzerland. Electronic address:
Background: Dry eye disease (DED) is caused by inflammation on the ocular surface and insufficient quality or production of the tear film. Due to various harmful environmental conditions, a gradual increase of DED cases has been reported.
Hypothesis/purpose: This study aims for a comprehensive in vitro pharmacological and phytochemical profiling of two different Buddleja officinalis Maxim.
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