and Regulate the Proliferation of Dermal Papilla Cells and Target the Gene.

Previous studies have demonstrated that could contribute to the proliferation of DPCs in vitro, but the upstream transcriptional regulatory mechanisms of remain largely unknown. This study aimed to investigate the upstream transcriptional regulators of to enhance our comprehension of the mechanism of action of the gene in ovine DPCs. Initially, the JASPAR (2024) software was used to predict the upstream target transcription factors for the gene. Subsequently, through RT-qPCR and a double luciferase reporter assay, the interaction between , , and was established, respectively. The results indicated that and were two highly reliable upstream transcription regulators for the gene. Additionally, we found that promoted the proliferation of DPCs by overexpressing in DPCs, and inhibited the proliferation of DPCs by overexpressing in DPCs. These findings are also consistent with the transcriptional regulation of by and , respectively. This study suggests that the effect of DPC proliferation in vitro by may regulated by the transcription factors and .