sp. nov., a bacterium isolated from mountain soil in the habitat of .

Int J Syst Evol Microbiol

Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650500, PR China.

Published: February 2024

AI Article Synopsis

  • - A new Gram-positive bacterial strain, WQ 127069, was discovered in the soil of Baima Snow Mountain, where endangered Yunnan snub-nosed monkeys live, and it underwent a comprehensive taxonomic analysis.
  • - Phylogenetic studies revealed that this strain is closely related to known strains in the genus, with high sequence similarity to PM10 and LMG 31456, and it exhibits specific genomic and biochemical characteristics, such as a G+C content of 45.6 mol% and MK-7 as its predominant quinone.
  • - The strain has a proposed optimal growth range of 20-35 °C, pH 7.0, and 0.5% NaCl,

Article Abstract

A novel Gram-positive strain WQ 127069 that was isolated from the soil of Baima Snow Mountain, a habitat of highly endangered Yunnan snub-nosed monkeys (), was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on the 16S rRNA gene sequences showed that the isolate belongs to the genus , showing 98.4 and 96.08 % sequence similarity to the type strains PM10 and LMG 31456, respectively. The G+C content of the genomic DNA of strain WQ127069 was 45.6 mol%. The predominant isoprenoid quinone was MK-7, and -diaminopimelic acid was present in peptidoglycan. The major cellular fatty acids were antiiso-C, iso-C and C. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmonomethylethanolamine. The whole genome average nucleotide identity and digital DNA-DNA hybridization values between strain WQ 127069 and strain PM10 were 93.2 and 52.5 %, respectively. Growth occurred at 5-40 °C (optimally at 20-35 °C), pH 6-8 (optimally at pH7.0) and with 0.5-2 % (w/v) NaCl (optimally at 0.5 %). On the basis of the taxonomic evidence, a novel species, sp. nov., is proposed. The type strain is WQ 127069 (=KCTC 43480=CCTCC AB 2022381).

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http://dx.doi.org/10.1099/ijsem.0.006260DOI Listing

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sp. nov., a bacterium isolated from mountain soil in the habitat of .

Int J Syst Evol Microbiol

February 2024

Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650500, PR China.

Article Synopsis
  • - A new Gram-positive bacterial strain, WQ 127069, was discovered in the soil of Baima Snow Mountain, where endangered Yunnan snub-nosed monkeys live, and it underwent a comprehensive taxonomic analysis.
  • - Phylogenetic studies revealed that this strain is closely related to known strains in the genus, with high sequence similarity to PM10 and LMG 31456, and it exhibits specific genomic and biochemical characteristics, such as a G+C content of 45.6 mol% and MK-7 as its predominant quinone.
  • - The strain has a proposed optimal growth range of 20-35 °C, pH 7.0, and 0.5% NaCl,
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