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Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report. | LitMetric

AI Article Synopsis

  • - The study introduces a new molecular-genetic method for detecting lung cancer using exosomes isolated from urine samples of 35 lung cancer patients and 40 healthy controls, measuring the expression ratio of MMP-1/CD63 and levels of two specific microRNAs (miRNA-21 and miRNA-486-5p).
  • - Results showed that the MMP-1/CD63 ratio was significantly higher in lung cancer patients compared to healthy individuals, and the sensitivity and specificity of MMP-1/CD63 and miRNA-486-5p varied by gender, with an impressive overall sensitivity and specificity when both markers were combined in a logistic regression model.
  • - The combination of these markers could potentially be used for early lung

Article Abstract

The present study describes a novel molecular-genetic method suitable for lung cancer (LC) screening in the work-place and at community health centers. Using urinary-isolated exosomes from 35 patients with LC and 40 healthy volunteers, the expression ratio of MMP-1/CD63, and the relative expression levels of both microRNA (miRNA)-21 and miRNA-486-5p were measured. MMP-1/CD63 expression ratio was significantly higher in patients with LC than in the healthy controls {1.342 [95% confidence interval (CI): 0.890-1.974] vs. 0.600 (0.490-0.900); P<0.0001}. The relative expression of miRNA-486-5p in male healthy controls was significantly different from that in female healthy controls, whereas there was no significant difference in miRNA-21. Receiver operating characteristic curve (ROC) analysis of MMP-1/CD63 showed 92.5% sensitivity and 54.3% specificity, whereas miRNA-486-5p showed 85% sensitivity and 70.8% specificity for men, and 70.0% sensitivity and 72.7% specificity for women. The logistic regression model used to evaluate the association of LC with the combination of MMP-1/CD63 and miRNA-486-5p revealed that the area under the ROC curve was 0.954 (95% CI: 0.908-1.000), and the model had 89% sensitivity and 88% specificity after adjusting for age, sex and smoking status. These data suggested that the combined analysis of MMP-1/CD63 and miRNA-486-5p in urinary exosomes may be used to detect patients with early-stage LC in the work-place and at community health centers, although confirmational studies are warranted.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10851336PMC
http://dx.doi.org/10.3892/ol.2024.14261DOI Listing

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