Polyphasic characterisation of (Gomont, 1892) Strunecky, Komárek & J.R.Johansen, 2013 (Oscillatoriales, Cyanobacteria) using a metabolomic approach as a complementary tool.

As a result of the continuous revision of cyanobacterial taxonomy, (Agardh) Trevisan ex Gomont, 1892 has been transferred to the genus (Gomont, 1892) Strunecky, Komárek & J.R.Johansen, 2013. This transfer was based on a single strain and literature data. In the present study, we revise the taxonomic position of by applying the classical approach of polyphasic taxonomy and additionally using metabolomics. Cyanobacterial strains identified as and (type species of the genus ) were used for comparative analyses. In addition, the taxonomic relationship between the species and was determined on the basis of polyphasic characteristics. Monitoring of the morphological variability of and strains showed a difference in the morphology concerning the ends of the trichomes, the shape of the apical cells, as well as the presence/absence of the calyptra and its shape. The performed TEM analysis of the thylakoid arrangement of the studied strains showed parietal arrangement of the thylakoids in the representatives of genus and fascicular arrangement in genus . Molecular genetic analyses, based on 16S rDNA, revealed grouping of the investigated strains in a separate clade. This clade is far from the subtree, which is very clearly formed by the representatives of the type species of genus , namely . The metabolomic analysis involving and strains identified 39 compounds that could be used as potential biochemical markers to distinguish the two cyanobacterial species. Based on the data obtained, we suggest changing of the current status of by restoring its previous appurtenance to the genus under the name (Agardh) Trevisan ex Gomont, 1892 and distinguishing this species from genus .